Department of Chemical and Biological Engineering, Chalmers University of Technology, Kemivägen 10, 41296 Göteborg, Sweden.
Sci Rep. 2013 Sep 25;3:2743. doi: 10.1038/srep02743.
Lipid bilayer membranes are among the most ubiquitous structures in the living world, with intricate structural features and a multitude of biological functions. It is attractive to recreate these structures in the laboratory, as this allows mimicking and studying the properties of biomembranes and their constituents, and to specifically exploit the intrinsic two-dimensional fluidity. Even though diverse strategies for membrane fabrication have been reported, the development of related applications and technologies has been hindered by the unavailability of both versatile and simple methods. Here we report a rapid prototyping technology for two-dimensional fluidic devices, based on in-situ generated circuits of phospholipid films. In this "lab on a molecularly thin membrane", various chemical and physical operations, such as writing, erasing, functionalization, and molecular transport, can be applied to user-defined regions of a membrane circuit. This concept is an enabling technology for research on molecular membranes and their technological use.
脂质双层膜是生命世界中最普遍存在的结构之一，具有复杂的结构特征和多种生物学功能。在实验室中重现这些结构是很有吸引力的，因为这可以模拟和研究生物膜及其成分的性质，并专门利用其内在的二维流动性。尽管已经报道了多种用于制造膜的策略，但由于缺乏通用且简单的方法，相关应用和技术的发展受到了阻碍。在这里，我们报告了一种基于磷脂膜原位生成电路的二维流体制备技术。在这个“分子级薄膜上的实验室”中，可以对膜电路的用户定义区域应用各种化学和物理操作，如写入、擦除、功能化和分子传输。这个概念是研究分子膜及其技术应用的一项使能技术。
