1] Department of Pharmacology, Keio University School of Medicine, Tokyo, Japan [2].
Sci Rep. 2013 Sep 25;3:2745. doi: 10.1038/srep02745.
To date, it has not been possible to measure microscopic diffusive water movements in epithelia and in the interstitial space of complex tissues and organs. Diffusive water movements are essential for life because they convey physiologically important small molecules, e.g. nutrients and signaling ligands throughout the extracellular space of complex tissues. Here we report the development of a novel method for the direct observation and quantitative analysis of water diffusion dynamics in a biologically organized tissue using Coherent Anti-Stokes Raman Scattering (CARS) microscopy. Using a computer simulation model to analyze the CARS O-H bond vibration data during H2O/D2O exchange in a 3D epithelial cyst, we succeeded in measuring the diffusive water permeability of the individual luminal and basolateral water pathways and also their response to hormonal stimulation. Our technique will be applicable to the measurement of diffusive water movements in other structurally complex and medically important tissues and organs.
迄今为止，还不可能测量上皮组织和复杂组织器官的细胞外间隙中的微观扩散水运动。扩散水运动对于生命至关重要，因为它们可以在复杂组织的细胞外空间中传递生理上重要的小分子，例如营养物质和信号配体。在这里，我们报告了一种使用相干反斯托克斯拉曼散射（CARS）显微镜直接观察和定量分析生物组织中水分扩散动力学的新方法。我们使用计算机模拟模型来分析在 3D 上皮囊中进行 H2O/D2O 交换期间的 CARS O-H 键振动数据，成功地测量了单个腔道和基底外侧水途径的扩散水渗透率，以及它们对激素刺激的反应。我们的技术将适用于测量其他结构复杂且具有医学重要性的组织和器官中的扩散水运动。
