Ganikhanov Feruz, Evans Conor L, Saar Brian G, Xie X Sunney
Department of Chemistry and Chemical Biology, Harvard University, 12 Oxford Street, Cambridge, Massachusetts 02138, USA.
Opt Lett. 2006 Jun 15;31(12):1872-4. doi: 10.1364/ol.31.001872.
We demonstrate a new approach to coherent anti-Stokes Raman scattering (CARS) microscopy that significantly increases the detection sensitivity. CARS signals are generated by collinearly overlapped, tightly focused, and raster scanned pump and Stokes laser beams, whose difference frequency is rapidly modulated. The resulting amplitude modulation of the CARS signal is detected through a lock-in amplifier. This scheme efficiently suppresses the nonresonant background and allows for the detection of far fewer vibrational oscillators than possible through existing CARS microscopy methods.
我们展示了一种用于相干反斯托克斯拉曼散射（CARS）显微镜的新方法，该方法显著提高了检测灵敏度。CARS信号由共线重叠、紧密聚焦并进行光栅扫描的泵浦激光束和斯托克斯激光束产生，其差频被快速调制。通过锁相放大器检测CARS信号产生的幅度调制。该方案有效地抑制了非共振背景，并允许检测比现有CARS显微镜方法所能检测到的少得多的振动振荡器。
