优化用于有髓组织相干反斯托克斯拉曼散射成像的激发参数。

Excitation parameters optimized for coherent anti-Stokes Raman scattering imaging of myelinated tissue.

机构信息

University of Calgary, Hotchkiss Brain Institute, Cumming School of Medicine, Department of Clinical, Canada.

University of Calgary, Department of Physiology and Pharmacology, Calgary, Alberta, Canada.

出版信息

J Biomed Opt. 2019 Apr;24(4):1-8. doi: 10.1117/1.JBO.24.4.046502.

DOI:10.1117/1.JBO.24.4.046502

PMID:31007003

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6990057/

Abstract

Coherent anti-Stokes Raman scattering (CARS) generates a strong label-free signal in the long wavenumber C─H stretching region. Lipid-rich myelinated tissues, such as brain and spinal cord, would appear to be ideal subjects for imaging with CARS laser-scanning microscopy. However, the highly ordered, biochemically complex, and highly scattering nature of such tissues complicate the use of the technique. A CARS microscopy approach is presented that overcomes the challenges of imaging myelinated tissue to achieve chemically and orientationally sensitive high-resolution images.

摘要

相干反斯托克斯拉曼散射（CARS）在长波数 C─H 伸缩区域产生强的无标记信号。富含脂质的有髓鞘组织，如脑和脊髓，似乎是用 CARS 激光扫描显微镜成像的理想对象。然而，这种组织具有高度有序、生化复杂和高度散射的特性，使得该技术的应用变得复杂。本文提出了一种 CARS 显微镜方法，克服了成像有髓鞘组织的挑战，实现了具有化学和取向敏感性的高分辨率图像。