University of Calgary, Hotchkiss Brain Institute, Cumming School of Medicine, Department of Clinical, Canada.
University of Calgary, Department of Physiology and Pharmacology, Calgary, Alberta, Canada.
J Biomed Opt. 2019 Apr;24(4):1-8. doi: 10.1117/1.JBO.24.4.046502.
Coherent anti-Stokes Raman scattering (CARS) generates a strong label-free signal in the long wavenumber C─H stretching region. Lipid-rich myelinated tissues, such as brain and spinal cord, would appear to be ideal subjects for imaging with CARS laser-scanning microscopy. However, the highly ordered, biochemically complex, and highly scattering nature of such tissues complicate the use of the technique. A CARS microscopy approach is presented that overcomes the challenges of imaging myelinated tissue to achieve chemically and orientationally sensitive high-resolution images.
相干反斯托克斯拉曼散射（CARS）在长波数 C─H 伸缩区域产生强的无标记信号。富含脂质的有髓鞘组织，如脑和脊髓，似乎是用 CARS 激光扫描显微镜成像的理想对象。然而，这种组织具有高度有序、生化复杂和高度散射的特性，使得该技术的应用变得复杂。本文提出了一种 CARS 显微镜方法，克服了成像有髓鞘组织的挑战，实现了具有化学和取向敏感性的高分辨率图像。
