Thornsberry C
Institutes for Microbiology Research, Nashville, Tennessee 37203.
Rev Infect Dis. 1990 Jan-Feb;12 Suppl 2:S218-22. doi: 10.1093/clinids/12.supplement_2.s218.
The National Committee for Clinical Laboratory Standards (NCCLS) has published a standard method (M11-A) and alternative methods (M17-P) for susceptibility testing of anaerobic bacteria. (These will be combined in the next edition of M11.) Even though M11-A describes the "standard reference" method, there is not agreement as to which method, if any, is the "best" method. In recent years NCCLS subcommittee members have realized that results obtained by microdilution or disk elution may vary markedly from those obtained with the reference agar dilution method. Furthermore, they have recognized the difficulty of relating in vitro susceptibility results to clinical success or failure. In response to these problems, the NCCLS convened a working group to consider recommendations about susceptibility testing. This group has concluded that for most individual patients a susceptibility test may not be required but that present methods can be useful for comparisons of the activity of various drugs and for surveillance of resistance patterns.
美国国家临床实验室标准委员会（NCCLS）已经发布了针对厌氧菌药敏试验的标准方法（M11 - A）和替代方法（M17 - P）。（这两种方法将在下一版的M11中合并。）尽管M11 - A描述了“标准参考”方法，但对于哪种方法（如果有的话）是“最佳”方法，并没有达成共识。近年来，NCCLS小组委员会成员已经意识到，微量稀释法或纸片洗脱法所获得的结果可能与参考琼脂稀释法所获得的结果有显著差异。此外，他们已经认识到将体外药敏结果与临床治疗成功或失败联系起来存在困难。为应对这些问题，NCCLS召集了一个工作组来考虑关于药敏试验的建议。该小组得出结论，对于大多数个体患者可能不需要进行药敏试验，但目前的方法对于比较各种药物的活性以及监测耐药模式可能是有用的。
