Scida Karen, Li Bingling, Ellington Andrew D, Crooks Richard M
Department of Chemistry and Biochemistry, Center for Nano- and Molecular Science and Technology, The University of Texas at Austin, 105 E. 24th St., Stop A5300, Austin, TX, 78712-1224, USA.
Anal Chem. 2013 Oct 15;85(20):9713-20. doi: 10.1021/ac402118a. Epub 2013 Sep 26.
We demonstrate the hybridization-induced fluorescence detection of DNA on an origami-based paper analytical device (oPAD). The paper substrate was patterned by wax printing and controlled heating to construct hydrophilic channels and hydrophobic barriers in a three-dimensional fashion. A competitive assay was developed where the analyte, a single-stranded DNA (ssDNA), and a quencher-labeled ssDNA competed for hybridization with a fluorophore-labeled ssDNA probe. Upon hybridization of the analyte with the fluorophore-labeled ssDNA, a linear response of fluorescence vs analyte concentration was observed with an extrapolated limit of detection <5 nM and a sensitivity relative standard deviation as low as 3%. The oPAD setup was also tested against OR/AND logic gates, proving to be successful in both detection systems.
我们展示了在基于折纸的纸质分析装置（oPAD）上通过杂交诱导的DNA荧光检测。通过蜡印和控制加热对纸质基底进行图案化处理，以三维方式构建亲水通道和疏水屏障。开发了一种竞争性检测方法，其中分析物（单链DNA，ssDNA）和淬灭剂标记的ssDNA与荧光团标记的ssDNA探针竞争杂交。当分析物与荧光团标记的ssDNA杂交时，观察到荧光与分析物浓度呈线性响应，外推检测限<5 nM，灵敏度相对标准偏差低至3%。oPAD装置还针对“或/与”逻辑门进行了测试，结果证明在两种检测系统中均成功。
