Locci Rosella, Nisolle Michelle, Angioni Stefano, Foidart Jean-Michel, Munaut Carine
Laboratory of Tumor and Development Biology, GIGA-Cancer, University of Liège, Tour de Pathologie (B23), Sart Tilman, B-4000 Liège, Belgium.
Reprod Biol Endocrinol. 2013 Sep 26;11:94. doi: 10.1186/1477-7827-11-94.
Endometrial cells, which are shed by retrograde menstruation, may aberrantly express molecules involved in invasion and migration, leading to endometriosis. The aim of this study was to investigate the expression of the laminin gamma 2 chain (LAMC2) in the tissues of women with and without endometriosis.
Endometrial biopsy specimens were collected from healthy volunteers and from endometriosis patients. Biopsy specimens from the corresponding endometriotic lesions were also collected. The expression of laminin gamma 2 chain was evaluated by immunohistochemistry and reverse transcription polymerase chain reaction (RT-PCR).
Endometrial tissue from women with or without endometriosis showed constitutive expression of LAMC2 mRNA throughout the menstrual cycle. A higher mRNA level was observed in ectopic endometrium (Ec) from women with endometriosis compared with eutopic endometrium (Eu) from women with endometriosis. Immunohistochemistry revealed a varied pattern of laminin gamma 2 chain expression, with increased epithelial expression in eutopic endometrium from women with endometriosis compared with those without endometriosis.
The altered expression of laminin gamma 2 chain in eutopic endometrium from women with endometriosis may provide new opportunities for diagnosis and treatment.
逆行月经排出的子宫内膜细胞可能异常表达参与侵袭和迁移的分子，从而导致子宫内膜异位症。本研究旨在调查层粘连蛋白γ2链（LAMC2）在有和没有子宫内膜异位症的女性组织中的表达情况。
从健康志愿者和子宫内膜异位症患者中收集子宫内膜活检标本。还收集了相应子宫内膜异位症病变的活检标本。通过免疫组织化学和逆转录聚合酶链反应（RT-PCR）评估层粘连蛋白γ2链的表达。
有或没有子宫内膜异位症的女性的子宫内膜组织在整个月经周期中均显示LAMC2 mRNA的组成性表达。与患有子宫内膜异位症的女性的在位子宫内膜（Eu）相比，患有子宫内膜异位症的女性的异位内膜（Ec）中观察到更高的mRNA水平。免疫组织化学显示层粘连蛋白γ2链表达模式各异，与没有子宫内膜异位症的女性相比，患有子宫内膜异位症的女性的在位子宫内膜上皮表达增加。
患有子宫内膜异位症的女性的在位子宫内膜中层粘连蛋白γ2链的表达改变可能为诊断和治疗提供新的机会。
