Minimally Invasive Urology Center, Shandong Provincial Hospital Affiliated to Shandong University, Jinan 250021, People's Republic of China.
Biomaterials. 2013 Dec;34(38):10258-66. doi: 10.1016/j.biomaterials.2013.09.027. Epub 2013 Sep 24.
The aim of this study was to develop a magnetic thermosensitive hydrogel as intravesical Bacillus Calmette-Guérin (BCG) delivery system, which was formulated with chitosan (CS), β-glycerophosphate (GP) and Fe3O4 magnetic nanoparticle (Fe3O4-MNP). The gelation time and magnetic response of the gel system were investigated. The morphology of the gel was displayed by scanning electron microscope. Frozen section examination was creatively employed for exhibiting the structure of the gel and determining its intravesical residence time. The antitumor effect and local immune activity of BCG loaded magnetic gel were evaluated. The flowing solution of CS/GP under room temperature could gelate rapidly at body temperature both in vitro and in vivo. The magnetic injectable hydrogels significantly prolonged intravesical BCG residence time under an applied magnetic field. In comparison to traditional BCG therapy for superficial bladder tumor, BCG delivered by the gel system induced a stronger Th1 immune response and revealed higher antitumor efficacy.
本研究旨在开发一种磁性温敏水凝胶作为膀胱内卡介苗（BCG）的给药系统，该系统由壳聚糖（CS）、β-甘油磷酸（GP）和四氧化三铁磁性纳米颗粒（Fe3O4-MNP）组成。考察了凝胶系统的凝胶时间和磁响应。扫描电子显微镜显示了凝胶的形态。创新性地采用冷冻切片检查来展示凝胶的结构并确定其在膀胱内的驻留时间。评价了载药磁性凝胶的抗肿瘤作用和局部免疫活性。在室温下流动的 CS/GP 溶液在体温和体内都能迅速凝胶化。在磁场作用下，磁性可注射水凝胶显著延长了膀胱内 BCG 的驻留时间。与传统的浅表膀胱癌 BCG 治疗相比，该凝胶系统递送的 BCG 诱导了更强的 Th1 免疫反应，并显示出更高的抗肿瘤疗效。
