Makshakova Olga N, Bogdanova Liliya R, Makarova Anastasiya O, Kusova Aleksandra M, Ermakova Elena A, Kazantseva Mariia A, Zuev Yuriy F
Kazan Institute of Biochemistry and Biophysics, FRC Kazan Scientific Center of RAS, Lobachevsky St., 2/31, 420111 Kazan, Russia.
HSE Tikhonov Moscow Institute of Electronics and Mathematics, Tallinskaya St., 34, 123458 Moscow, Russia.
Polymers (Basel). 2022 Sep 28;14(19):4071. doi: 10.3390/polym14194071.
During the last few decades, polysaccharide hydrogels attract more and more attention as therapeutic protein delivery systems due to their biocompatibility and the simplicity of the biodegradation of natural polymers. The protein retention by and release from the polysaccharide gel network is regulated by geometry and physical interactions of protein with the matrix. In the present work, we studied the molecular details of interactions between κ-carrageenan and three lipases, namely the lipases from , , and -which differ in their size and net charge-upon protein immobilization in microparticles of polysaccharide gel. The kinetics of protein release revealed the different capability of κ-carrageenan to retain lipases, which are generally negatively charged; that was shown to be in line with the energy of interactions between polysaccharides and positively charged epitopes on the protein surface. These data create a platform for the novel design of nanocarriers for biomedical probes of enzymatic origin.
在过去几十年中，多糖水凝胶作为治疗性蛋白质递送系统越来越受到关注，这是由于其生物相容性以及天然聚合物生物降解的简易性。蛋白质在多糖凝胶网络中的保留和释放受蛋白质与基质的几何结构和物理相互作用调节。在本研究中，我们研究了κ-卡拉胶与三种脂肪酶之间相互作用的分子细节，这三种脂肪酶分别来自、和，它们在大小和净电荷方面存在差异，将其固定在多糖凝胶微粒中时会与蛋白质相互作用。蛋白质释放动力学揭示了κ-卡拉胶保留脂肪酶的不同能力，这些脂肪酶通常带负电荷；结果表明这与多糖和蛋白质表面带正电荷表位之间的相互作用能量一致。这些数据为酶源生物医学探针纳米载体的新型设计创造了一个平台。
