State Key Lab of Urban and Regional Ecology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China.
Environ Pollut. 2014 Jan;184:247-53. doi: 10.1016/j.envpol.2013.08.038. Epub 2013 Sep 24.
The abundance and distribution of antibiotics and antibiotic resistance genes (ARGs) in soils from six parks using reclaimed water in Beijing, China, were characterized. Three classes of commonly used antibiotics (tetracycles, quinolones, and sulfonamides) were analyzed by liquid chromatography-tandem mass spectrometry (LC-MS/MS). The highest concentrations of tetracyclines and quinolones were 145.2 μg kg(-1) and 79.2 μg kg(-1), respectively. Detected tetG, tetW, sulI, and sulII genes were quantified by quantitative PCR. ARGs exhibited various abundances for different park soils. The integrase gene (intI1) as an indicator of horizontal gene transfer potential was also detected in high abundance, and had significant positive correlation with tetG, sulI, and sulII genes, suggesting that intI1 may be involved in ARGs dissemination. Both sulII and intI1 clones had high homology with some classes of pathogenic bacteria, such as Klebsiella oxytoca, Acinetobacter baumannii, Shigella flexneri, which could trigger potential public health concern.
采用液相色谱-串联质谱法（LC-MS/MS）分析了中国北京 6 个使用再生水的公园土壤中抗生素和抗生素抗性基因（ARGs）的丰度和分布。检测到的 tetG、tetW、sulI 和 sulII 基因通过定量 PCR 进行定量。不同公园土壤中 ARGs 的丰度存在差异。作为水平基因转移潜力的指示物的整合酶基因（intI1）也以高丰度存在，并且与 tetG、sulI 和 sulII 基因呈显著正相关，表明 intI1 可能参与了 ARGs 的传播。sulII 和 intI1 克隆与一些病原菌（如产酸克雷伯氏菌、鲍曼不动杆菌、福氏志贺菌）具有高度同源性，这可能引发潜在的公共卫生关注。
