环境条件对重组大肠杆菌木糖发酵的影响
Effects of environmental conditions on xylose fermentation by recombinant Escherichia coli.
作者信息
Ohta K, Alterthum F, Ingram L O
机构信息
Department of Microbiology and Cell Science, University of Florida, Gainesville 32611.
出版信息
Appl Environ Microbiol. 1990 Feb;56(2):463-5. doi: 10.1128/aem.56.2.463-465.1990.
Abstract
In batch fermentations, optimal conversion of xylose to ethanol by recombinant Escherichia coli was obtained under the following conditions: 30 to 37 degrees C, pH 6.4 to 6.8, 0.1 to 0.2 M potassium phosphate buffer, and xylose concentrations of 8% or less. A yield of 39.2 g of ethanol per liter (4.9% ethanol by volume) was observed with 80 g of xylose per liter, equivalent to 96% of the maximum theoretical yield. Maximal volumetric productivity was 0.7 g of ethanol per liter per h in batch fermentations and 30 g of ethanol per liter per h in concentrated cell suspensions (analogous to cell recycling).
摘要
在分批发酵中，重组大肠杆菌将木糖最佳转化为乙醇的条件如下：30至37摄氏度、pH值6.4至6.8、0.1至0.2M磷酸钾缓冲液以及木糖浓度为8%或更低。每升80克木糖时观察到乙醇产量为每升39.2克（体积分数为4.9%乙醇），相当于最大理论产量的96%。分批发酵中的最大体积产率为每升每小时0.7克乙醇，在浓缩细胞悬浮液中（类似于细胞循环）为每升每小时30克乙醇。
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