Institute of Biochemistry, School of Medicine, University of Belgrade, Belgrade, Serbia.
Planta Med. 2013 Nov;79(16):1516-24. doi: 10.1055/s-0033-1350809. Epub 2013 Sep 26.
Sideritis scardica (mountain tea) is an endemic plant on the Balkan Peninsula traditionally used for treating different conditions, mainly of inflammatory nature. This study was aimed to examine the cytotoxic activity of different S. scardica extracts against the rat glioma C6 line and rat astrocytes in primary culture. The obtained data revealed that diethyl ether (extract 2) and ethyl acetate (extract 3) extracts of S. scardica exerted a cytotoxic effect on C6 rat glioma cells. Diethyl ether extract induced an increase in reactive oxygen species production, leading to apoptotic and autophagic cell death. Ethyl acetate extract induced G2 M cell cycle arrest and autophagy. None of the tested extracts was cytotoxic to rat astrocytes in primary culture. Cytotoxic effects of S. scardica extracts were, at least in part, mediated by their flavonoid constituents apigenin and luteolin that, when applied alone, induced cell cycle arrest, apoptosis, and autophagy.
Scardica（山茶）是巴尔干半岛特有的植物，传统上用于治疗多种疾病，主要是炎症性疾病。本研究旨在研究不同 Scardica 提取物对大鼠神经胶质瘤 C6 细胞系和原代培养大鼠星形胶质细胞的细胞毒性作用。研究结果表明，Scardica 的乙醚（提取物 2）和乙酸乙酯（提取物 3）提取物对大鼠神经胶质瘤 C6 细胞具有细胞毒性作用。乙醚提取物可诱导活性氧的产生，导致细胞凋亡和自噬性细胞死亡。乙酸乙酯提取物诱导 G2/M 细胞周期阻滞和自噬。在原代培养的大鼠星形胶质细胞中，没有一种测试提取物具有细胞毒性。Scardica 提取物的细胞毒性作用至少部分是由其类黄酮成分芹菜素和木犀草素介导的，当单独应用时，它们诱导细胞周期阻滞、细胞凋亡和自噬。
