, CNR-IAMC-Taranto, Via Roma 3, 74100, Taranto, Italy.
Environ Sci Pollut Res Int. 2014 Feb;21(3):2378-2385. doi: 10.1007/s11356-013-2049-3. Epub 2013 Sep 27.
Microorganisms play an indispensable role in the ecological functioning of marine environment. Some species are sensitive while others are insensitive for a specific pollutant. The aim of this work is a preliminary study of the quantitative and qualitative distribution of cultivable vibrios in sediments and water samples characterized by different toxicity levels. For 1 year, in three suitably selected sampling stations of Mar Piccolo in Taranto (Ionian Sea, Italy), we have evaluated the toxicity level by Microtox® system, vibrios, total, and fecal coliform densities. The results of the Microtox® tests showed sediments characterized by an elevated level of toxicity, while the interstitial water of the same sites always showed biostimulatory phenomenon. The quantitative results show that vibrios and coliforms are more abundant in water than in sediment samples. The most often isolated strains were: Vibrio alginolyticus, Vibrio mediterranei, Vibrio metschinkovii, and Vibrio splendidus II. This work is the first example of study on the distribution of Vibrio species related to toxicity evaluation conducted by the Microtox® bioassay. The results show the different distribution of Vibrionaceae in two environmental matrices analyzed and characterized by different levels of toxicity.
微生物在海洋环境的生态功能中起着不可或缺的作用。有些物种对特定污染物敏感，而有些则不敏感。本工作旨在初步研究具有不同毒性水平的沉积物和水样中可培养弧菌的定量和定性分布。在意大利塔兰托（伊奥尼亚海）的马雷皮科洛（Mar Piccolo）的三个适当选择的采样站，我们通过 Microtox®系统、弧菌、总大肠菌群和粪大肠菌群密度来评估毒性水平。Microtox®测试的结果表明沉积物具有较高的毒性水平，而同一地点的间隙水则始终表现出生物刺激现象。定量结果表明，弧菌和大肠菌群在水样中的丰度高于沉积物样品。最常分离到的菌株为：溶藻弧菌、地中海弧菌、梅奇科夫斯基弧菌和灿烂弧菌 II。本工作是首次应用 Microtox®生物测定法研究与毒性评价相关的弧菌种分布的实例。结果表明，在两种环境基质中的 Vibrionaceae 分布不同，这两种基质具有不同的毒性水平。
