Giarratana Filippo, Ziino Graziella, D'Andrea Valerio, Panebianco Antonio, Giuffrida Alessandro
Department of Veterinary Science, University of Messina, Italy.
Ital J Food Saf. 2018 Apr 9;7(1):6997. doi: 10.4081/ijfs.2018.6997. eCollection 2018 Mar 31.
In the last few years, the consumption of fish eggs has increased rapidly, finding widespread use also in mass catering. This increase has involved also those of the Peter's fish (). Females of this species, by their reproductive characteristics, have highly developed gonads in different periods of the year, making the raw material easy to find. The aim of the present study was to perform a quality assessment of ovaries regularly commercialized for human consumption. A total number of 34 samples, divided in fresh (11) and frozen (23), were processed for microbiological characterization, parasitological and histological evaluations. Fresh and frozen samples have significant (P<0.01) differences in total bacterial charge, with values of 4.75±0.5 Log CFU/g and 3.65±0.7 Log CFU/g respectively. The mean value of was 2.58±0.7 Log CFU/g in fresh products, while 52.17% (12) of frozen samples reported loads of <1 Log CFU/g. No spp. and were found. spp. was detected in two frozen sample (with loads of 2.2 and <1 Log CFU/g) and in 5 fresh ovaries with value ranged from 1.70 to 3.48 Log CFU/g. spp. was found in 4 (36.36%) and 3 (13.04%) of fresh and frozen samples respectively, with loads always <1 Log CFU/g. All 31 strains isolated, were identified as , and 61.29% (19) of them was positive for the ToxRS factor and 6.45% (2) for ToxR. The 47.06% (16) of total samples showed infestations by larvae of Type 1 in the serous and inside the ovary. In this last case, histologically it was found to be free larvae. This study attested satisfactory hygiene conditions for ovaries currently marked for human consumption. The presence of potentially pathogenic strains of and spp., but above all the frequent infestation by larvae, represent a potentially hazard for the consumer.
在过去几年中,鱼卵的消费量迅速增加,在大规模餐饮中也得到广泛应用。这种增长也涉及彼得氏鱼()的鱼卵。该物种的雌性由于其生殖特征,在一年中的不同时期性腺高度发达,使得原材料易于获取。本研究的目的是对定期商业化用于人类消费的卵巢进行质量评估。总共34个样本,分为新鲜样本(11个)和冷冻样本(23个),进行了微生物特征分析、寄生虫学和组织学评估。新鲜样本和冷冻样本在总细菌负荷上有显著差异(P<0.01),分别为4.75±0.5 Log CFU/g和3.65±0.7 Log CFU/g。新鲜产品中的平均值为2.58±0.7 Log CFU/g,而52.17%(12个)的冷冻样本报告负荷<1 Log CFU/g。未发现 spp. 和 。在两个冷冻样本(负荷分别为2.2和<1 Log CFU/g)以及5个新鲜卵巢中检测到 spp.,其值范围为1.70至3.48 Log CFU/g。在新鲜样本的4个(36.36%)和冷冻样本的3个(13.04%)中分别发现了 spp.,负荷均<1 Log CFU/g。分离出的所有31株 菌株均被鉴定为 ,其中61.29%(19株)对ToxRS因子呈阳性,6.45%(2株)对ToxR呈阳性。47.06%(16个)的总样本在浆液和卵巢内部显示有1型幼虫感染。在最后这种情况下,组织学检查发现为游离幼虫。本研究证明了目前标记用于人类消费的卵巢具有令人满意的卫生条件。 spp. 和 spp. 潜在致病菌株的存在,尤其是 幼虫的频繁感染,对消费者构成潜在危害。