菲律宾检测到的肠道病毒 68 型的分子进化。
Molecular evolution of enterovirus 68 detected in the Philippines.
机构信息
Tohoku University Graduate School of Medicine, Sendai, Japan.
出版信息
PLoS One. 2013 Sep 20;8(9):e74221. doi: 10.1371/journal.pone.0074221. eCollection 2013.
BACKGROUND
Detection of Enterovirus 68 (EV68) has recently been increased. However, underlying evolutionary mechanism of this increasing trend is not fully understood.
METHODS
Nasopharyngeal swabs were collected from 5,240 patients with acute respiratory infections in the Philippines from June 2009 to December 2011. EV68 was detected by polymerase chain reaction (PCR) targeting for 5' untranslated region (5'UTR), viral protein 1 (VP1), and VP4/VP2. Phylogenetic trees were generated using the obtained sequences.
RESULTS
Of the 5,240 tested samples, 12 EV68 positive cases were detected between August and December in 2011 (detection rate, 0.23%). The detection rate was higher among inpatients than outpatients (p<0.0001). Among VP1 sequences detected from 7 patients in 2011, 5 in lineage 2 were diverged from those detected in the Philippines in 2008, however, 2 in lineage 3 were not diverged from strains detected in the Philippines in 2008 but closely associated with strains detected in the United States. Combined with our previous report, EV68 occurrences were observed twice in the Philippines within the last four years.
CONCLUSIONS
EV68 detections might be occurring in cyclic patterns, and viruses might have been maintained in the community while some strains might have been newly introduced.
背景
最近，肠道病毒 68 型（EV68）的检测有所增加。然而，这种上升趋势的潜在进化机制尚不完全清楚。
方法
从 2009 年 6 月至 2011 年 12 月，从菲律宾 5240 例急性呼吸道感染患者中采集鼻咽拭子。通过聚合酶链反应（PCR）针对 5'非翻译区（5'UTR）、病毒蛋白 1（VP1）和 VP4/VP2 检测 EV68。使用获得的序列生成系统发育树。
结果
在 5240 个测试样本中，2011 年 8 月至 12 月期间检测到 12 例 EV68 阳性病例（检出率为 0.23%）。住院患者的检出率高于门诊患者（p<0.0001）。在 2011 年检测到的 7 例患者的 VP1 序列中，5 例属于 2 谱系的病毒与 2008 年在菲律宾检测到的病毒有差异，然而，3 谱系中的 2 例与 2008 年在菲律宾检测到的病毒没有差异，但与在美国检测到的病毒密切相关。结合我们之前的报告，在过去四年中，EV68 在菲律宾出现了两次。
结论
EV68 的检测可能呈周期性发生，病毒可能在社区中持续存在，而某些毒株可能是新引入的。
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