Gallagher G, Stimson W H, Findlay J, al-Azzawi F
Immunology Division, University of Strathclye, Todd Centre, Glasgow.
Cancer Immunol Immunother. 1990;31(1):49-52. doi: 10.1007/BF01742495.
Human peripheral blood mononuclear cells develop a powerful lytic capacity when cultured in vitro with interleukin-2 (IL-2), becoming lymphokine-activated killer cells (LAK cells). As part of an investigation into means of influencing this process, the effect of other cytokines has been examined. In this study we describe the ability of interleukin-6 (IL-6) to regulate the induction and function of human LAK cells. The results show that substitution of IL-6 for IL-2 did not lead to the development of functional LAK cells, nor was IL-6 able to alter the lytic capacity of established LAK cells. However, when IL-6 was included with IL-2 during the induction phase of the LAK cells, the resulting cells displayed considerably greater lytic activity than those prepared with IL-2 alone. This effect was IL-6 dose-related. These results indicate that LAK cell development may be positively regulated in vitro; the implications of this observation for the clinical usage of LAK cells are discussed.
人外周血单个核细胞在体外与白细胞介素-2（IL-2）一起培养时会产生强大的溶解能力，成为淋巴因子激活的杀伤细胞（LAK细胞）。作为影响这一过程方法研究的一部分，已检测了其他细胞因子的作用。在本研究中，我们描述了白细胞介素-6（IL-6）调节人LAK细胞诱导和功能的能力。结果表明，用IL-6替代IL-2不会导致功能性LAK细胞的产生，IL-6也不能改变已建立的LAK细胞的溶解能力。然而，当在LAK细胞的诱导阶段将IL-6与IL-2一起加入时，所产生的细胞显示出比单独用IL-2制备的细胞显著更高的溶解活性。这种效应与IL-6剂量相关。这些结果表明，LAK细胞的发育在体外可能受到正向调节；讨论了这一观察结果对LAK细胞临床应用的意义。
