Van Oers M H, Van der Heyden A A, Aarden L A
Renal Transplant Unit, Academic Medical Centre (AMC), Amsterdam, The Netherlands.
Clin Exp Immunol. 1988 Feb;71(2):314-9.
Hybridoma growth factor (HGF) is a 20-25 kD protein, supporting the growth of hybridoma cells in vitro and capable of replacing feeder cells. It was shown to be produced by human monocytes and a number of cultured cell lines. Recently, HGF was found to be identical to interferon-beta 2 or 26 kD protein and BSF-2, and was renamed interleukin 6 (IL-6). Using a sensitive bio-assay we were able to measure IL-6 activity in the serum and urine of healthy volunteers and renal transplant recipients. Low levels of IL-6 were present in the serum but not in the urine of healthy individuals. In contrast, both serum and urine of renal transplant recipients contained high levels of IL-6 directly after transplantation and during acute rejection episodes. On the basis of kinetic studies of the IL-6 response, it is concluded that serial measurement of IL-6, especially in urine, may be of value in monitoring renal transplant recipients. Moreover, the sensitivity of the bioassay will allow for detailed studies as to the biological significance of IL-6 in health and disease.
杂交瘤生长因子（HGF）是一种20 - 25千道尔顿的蛋白质，可在体外支持杂交瘤细胞的生长并能够替代饲养细胞。已证明它由人单核细胞和一些培养细胞系产生。最近，发现HGF与干扰素-β2或26千道尔顿蛋白质以及BSF-2相同，并被重新命名为白细胞介素6（IL-6）。使用一种灵敏的生物测定法，我们能够测量健康志愿者和肾移植受者血清及尿液中的IL-6活性。健康个体的血清中存在低水平的IL-6，但尿液中没有。相反，肾移植受者在移植后即刻及急性排斥反应期间，血清和尿液中均含有高水平的IL-6。基于对IL-6反应的动力学研究，得出结论：连续测量IL-6，尤其是尿液中的IL-6，可能对监测肾移植受者有价值。此外这种生物测定法的灵敏度将有助于对IL-6在健康和疾病中的生物学意义进行详细研究。
