Hira Arpona, Dey Shubhra Kanti, Howlader Md Sariful Islam, Ahmed Arif, Hossain Hemayet, Jahan Ismet Ara
Pharmacy Discipline, Life Science School, Khulna University, Khulna-9208, Bangladesh.
Asian Pac J Trop Biomed. 2013 Oct;3(10):798-805. doi: 10.1016/S2221-1691(13)60158-6. Epub 2013 Sep 4.
To investigate the inflammatory and antioxidant activities of ethanolic extract of aerial part of Vernonia patula (Dryand.) Merr (EAV).
The anti-inflammatory activity of EAV was studied using carrageenan and histamine-induced rat paw edema test at different doses (100, 200 and 400 mg/kg body weight). DPPH free radical scavenging, nitric oxide scavenging, reducing power and Fe(2+) ion chelating ability were used for determining antioxidant activities.
The EAV, at the dose of 400 mg/kg, showed a significant anti-inflammatory activity (P<0.01) both in the carrageenan and histamine-induced oedema test models in rats, showing 62.86% and 64.42% reduction in the paw volume comparable to that produced by the standard drug indomethacin (67.26% and 66.01%) at 5 h respectively. In DPPH free radical scavenging test, IC50 value for EAV was found fairly significant 36.59 μg/mL when compared to the IC50 value of the reference standards ascorbic acid 8.97 μg/mL. The IC50 values of the extract and ascorbic acid were 47.72 and 12.39 μg/mL, respectively in nitric oxide scavenging assay. The IC50 value of the EAV (33.59 μg/mL) as percentage of Fe(2+) ion chelating ability was also found significant compared to that of EDTA (9.16 μg/mL). The maximum absorbance for reducing power assay was found to be 1.928 at 100 μg/mL when compared to 2.449 for standard ascorbic acid. The total phenolic content was 198.81 mg/g of gallic acid equivalent. Acute toxicity test showed that the plant might be safe for pharmacological uses up to a dose level of 3 200 mg/kg of body weight in rats.
Therefore, the obtained results suggest the acute anti-inflammatory and antioxidant activities of the EAV and thus provide the scientific basis for the traditional uses of this plant part as a remedy for inflammations.
研究蟛蜞菊地上部分乙醇提取物（EAV）的抗炎和抗氧化活性。
采用角叉菜胶和组胺诱导的大鼠足跖水肿试验，在不同剂量（100、200和400mg/kg体重）下研究EAV的抗炎活性。采用DPPH自由基清除、一氧化氮清除、还原能力和Fe(2+)离子螯合能力来测定抗氧化活性。
在大鼠角叉菜胶和组胺诱导的水肿试验模型中，400mg/kg剂量的EAV均表现出显著的抗炎活性（P<0.01），在5小时时足跖体积分别减少62.86%和64.42%，与标准药物吲哚美辛（分别为67.26%和66.01%）相当。在DPPH自由基清除试验中，EAV的IC50值为36.59μg/mL，与参考标准抗坏血酸的IC50值8.97μg/mL相比，具有相当显著的差异。在一氧化氮清除试验中，提取物和抗坏血酸的IC50值分别为47.72和12.39μg/mL。EAV的IC50值（33.59μg/mL）在Fe(2+)离子螯合能力方面也与EDTA（9.16μg/mL）相比具有显著差异。在还原能力试验中，100μg/mL时的最大吸光度为1.928，而标准抗坏血酸为2.449。总酚含量为198.81mg/g没食子酸当量。急性毒性试验表明，该植物在大鼠体重剂量高达3200mg/kg时可能对药理用途是安全的。
因此，所得结果表明EAV具有急性抗炎和抗氧化活性，从而为该植物部分作为炎症治疗药物的传统用途提供了科学依据。
