Department of Biological Sciences, College of Basic Sciences and Humanities, G. B. Pant University of Agriculture and Technology, Pantnagar, Uttarakhand, India.
Department of Microbiology, College of Basic Sciences and Humanities, G. B. Pant University of Agriculture and Technology, Pantnagar, Uttarakhand, India.
World J Microbiol Biotechnol. 2018 Jul 6;34(8):111. doi: 10.1007/s11274-018-2493-9.
Centella asiatica (L.) Urban is a highly considered medicinal plant owing to its secondary metabolites asiaticoside, madecassoside, asiatic acid, and madecassic acid. The asiaticoside, one of the most important constituents of the plant, is a triterpenoid saponin having memory enhancement property. Given its medicinal properties, we isolated and characterized endophytic fungi from this plant with the aim to screen these microorganisms for asiaticoside production. In total, we isolated 13 endophytic fungi from the leaves of the plant, out of which one of the isolates produced asiaticoside. This asiaticoside producing isolate was identified as Colletotrichum gloeosporioides by internal transcribed spacer-based rDNA sequencing. The presence of asiaticoside in ethyl acetate extract of C. gloeosporioides was confirmed by LC-MS. The production of asiaticoside measured in relation to incubation time and subculture generation revealed presence of 62.29 ± 3.36 µg/100 mL of asiaticoside by C. gloeosporioides on the 15th day in first subculture generation followed by a decrease in subsequent generations. A similar trend was also shown by yield and growth curve of C. gloeosporioides. The asiaticoside production and yield were found to be positively correlated. This paper reported the production of asiaticoside by an endophytic fungus C. gloeosporioides for the first time. The present findings definitely provide an impetus to the production of asiaticoside by utilizing the endophytic source. Chemical compound studied in this article: Asiaticoside (PubChemCID: 108062).
积雪草（Centella asiatica（L.）Urban）是一种备受推崇的药用植物，因其含有多种次生代谢产物，如积雪草酸、羟基积雪草酸、积雪草苷和羟基积雪草苷等。其中，积雪草苷是该植物最重要的成分之一，是一种具有增强记忆功能的三萜皂苷。鉴于其药用特性，我们从该植物中分离并鉴定了内生真菌，旨在筛选这些微生物以生产积雪草苷。总共从植物叶片中分离出 13 株内生真菌，其中一株能产生积雪草苷。通过基于内部转录间隔区（ITS）的 rDNA 测序，该产生积雪草苷的内生真菌被鉴定为胶孢炭疽菌（Colletotrichum gloeosporioides）。通过 LC-MS 证实了胶孢炭疽菌乙酸乙酯提取物中存在积雪草苷。通过测量与培养时间和传代培养相关的积雪草苷产量，在第一代传代培养的第 15 天，C. gloeosporioides 产生的积雪草苷含量为 62.29±3.36μg/100mL，随后产量逐渐下降。C. gloeosporioides 的生长曲线和产量也呈现出类似的趋势。积雪草苷的产量和生成量呈正相关。本文首次报道了内生真菌胶孢炭疽菌（C. gloeosporioides）产生积雪草苷。这些发现肯定为利用内生来源生产积雪草苷提供了动力。本文研究的化合物：积雪草苷（PubChemCID：108062）。
