Giri D K, Chaudhuri M K, Jayashankar R, Neelaram G S, Jayaraman S, Talwar G P
National Institute of Immunology, New Delhi, India.
Exp Mol Pathol. 1990 Feb;52(1):54-62. doi: 10.1016/0014-4800(90)90058-l.
Histopathological changes induced by immune responses generated against luteinizing hormone releasing hormone (LHRH) were studied in adult male Wistar rats. Active immunization with a semisynthetic anti-LHRH vaccine, LHRH D-Lys6, (10, 40, and 80 micrograms/animal) conjugated with diphtheria toxoid, produced bioeffective antibodies as indicated by significant reduction in circulating testosterone levels. At the 14th week after immunization the animals were sacrificed and reproductive organs were evaluated. These organs were studied for histopathological changes and compared with those of nonimmunized control rats. Marked hypoplastic changes were observed in the genital organs. Testicular changes such as arrest of spermatogenesis at the spermatocyte level and atrophic changes in the interstitial Leydig cells were noticed in treated animals. Similarly attenuation of secretory epithelial cells with substantial increase in the stromal tissues was observed in the prostate and seminal vesicles. The current observation suggests the possible usefulness of this anti-LHRH vaccine under clinical conditions where reduction in androgenic response is desired as in the case of hormone-dependent prostatic carcinoma.
在成年雄性Wistar大鼠中研究了针对促黄体生成素释放激素（LHRH）产生的免疫反应所诱导的组织病理学变化。用与白喉类毒素结合的半合成抗LHRH疫苗LHRH D-Lys6（10、40和80微克/只动物）进行主动免疫，循环睾酮水平显著降低表明产生了生物活性抗体。免疫后第14周处死动物并评估生殖器官。对这些器官进行组织病理学变化研究，并与未免疫的对照大鼠进行比较。在生殖器官中观察到明显的发育不全变化。在接受治疗的动物中，注意到睾丸变化，如精子发生在精母细胞水平停滞以及间质Leydig细胞萎缩。同样，在前列腺和精囊中观察到分泌上皮细胞减少，间质组织大量增加。目前的观察结果表明，在需要降低雄激素反应的临床情况下，如激素依赖性前列腺癌，这种抗LHRH疫苗可能有用。
