Lam H C, Takahashi K, Ghatei M A, Bloom S R
Department of Medicine, Royal Postgraduate Medical School, Hammersmith Hospital, London, England.
FEBS Lett. 1990 Feb 12;261(1):184-6. doi: 10.1016/0014-5793(90)80666-7.
Endothelin-like immunoreactivity was detected in human milk at a concentration of 6.8 +/- 1.6 pmol/l (mean +/- SEM; n = 16) using a highly sensitive radioimmunoassay. Gel filtration and fast protein liquid chromatography (FPLC) verified the identity of the endothelin. FPLC revealed 4 peaks, one eluting just after the void volume, and the other three in the positions of endothelin-1, -2, and -3, respectively.
采用高灵敏度放射免疫分析法，在人乳中检测到内皮素样免疫反应性，其浓度为6.8±1.6皮摩尔/升（平均值±标准误；n = 16）。凝胶过滤和快速蛋白质液相色谱法（FPLC）证实了内皮素的特性。FPLC显示有4个峰，一个在空体积后洗脱，另外三个分别在内皮素-1、-2和-3的位置。
