培养的气道上皮细胞合成内皮素。

Formation of endothelin by cultured airway epithelial cells.

作者信息

Black P N, Ghatei M A, Takahashi K, Bretherton-Watt D, Krausz T, Dollery C T, Bloom S R

机构信息

Department of Clinical Pharmacology, Royal Postgraduate Medical School, London, England.

出版信息

FEBS Lett. 1989 Sep 11;255(1):129-32. doi: 10.1016/0014-5793(89)81075-0.

DOI:10.1016/0014-5793(89)81075-0

PMID:2676594

Abstract

Immunoreactivity to endothelin was detected in conditioned culture medium from both canine and porcine tracheal epithelial cells. Gel permeation chromatography and fast protein liquid chromatography were used to confirm the identity of the endothelin. The two peaks demonstrated on fast protein liquid chromatography co-eluted with endothelin 1 and endothelin 3 respectively.

摘要

在犬和猪气管上皮细胞的条件培养基中检测到了对内皮素的免疫反应性。采用凝胶渗透色谱法和快速蛋白质液相色谱法来确认内皮素的特性。快速蛋白质液相色谱法上显示的两个峰分别与内皮素1和内皮素3共洗脱。