对分离的小鼠胰岛β细胞中葡萄糖依赖性电活动和ATP调节的钾电流进行同步记录。

Simultaneous recordings of glucose dependent electrical activity and ATP-regulated K(+)-currents in isolated mouse pancreatic beta-cells.

作者信息

Smith P A, Ashcroft F M, Rorsman P

机构信息

University Laboratory of Physiology, Oxford University, England.

出版信息

FEBS Lett. 1990 Feb 12;261(1):187-90. doi: 10.1016/0014-5793(90)80667-8.

DOI:10.1016/0014-5793(90)80667-8

PMID:2407553

Abstract

Membrane potential and membrane currents were recorded from single mouse pancreatic beta-cells using the perforated patch whole-cell recording technique at 30 degrees C. Single beta-cells maintained in primary tissue culture exhibited glucose-dependent electrical activity similar to that reported for freshly isolated intact islets. The resting input conductance (5.1 +/- 0.9 nS) was determined by ATP-regulated K+ (KATP) channels as it was blocked by 1 mM tolbutamide. 8 mM glucose decreased the input conductance by 80%. The input conductance at -70 mV was of a similar value during the plateau phase and during the silent phase of electrical activity in 8 mM glucose. This suggests that oscillations of KATP channel activity do not underlie the slow waves.

摘要

在30℃下，使用穿孔膜片全细胞记录技术从小鼠单个胰腺β细胞记录膜电位和膜电流。维持在原代组织培养中的单个β细胞表现出葡萄糖依赖性电活动，类似于新鲜分离的完整胰岛所报道的电活动。静息输入电导（5.1±0.9 nS）由ATP调节的钾通道（KATP）决定，因为它被1 mM甲苯磺丁脲阻断。8 mM葡萄糖使输入电导降低80%。在8 mM葡萄糖条件下，电活动的平台期和静息期-70 mV时的输入电导值相似。这表明KATP通道活性的振荡不是慢波的基础。