Biomaterials and Tissue engineering, Eastman Dental Institute, University College London, 256 Grays's Inn Road, London WC1X 8LD, UK.
Bone. 2013 Dec;57(2):405-12. doi: 10.1016/j.bone.2013.09.012. Epub 2013 Sep 26.
The osteogenic and osseointegrative potential of a small molecule was examined to assess its usefulness in regenerative procedures. Purmorphamine was used to stimulate bone growth and repair in an in vitro cell-based assay and an in vivo chick embryo CAM-assay with and without the presence of an implant. Purmorphamine adhered to precipitated hydroxyapatite coating, could activate the sonic hedgehog pathway and thereby stimulated osteodifferentiation. Porous calcium phosphate beads were used to deliver this small molecule in vivo and showed that purmorphamine increased the trabecular bone to bone area significantly. The assay showed purmorphamine failed to induce any significant difference in osseointegration on titanium coated PTFE implants. This suggests that, while a small molecule can enhance osteogenesis and might be useful in regenerative procedures, it failed to enhance the osseointegration of a Ti coated implant, suggesting that this sort of stimulation might be useful for enhancing bone regeneration where bone loss due to disease exists, but not for enhancing early stability of an implant.
研究了一种小分子的成骨和骨整合潜力，以评估其在再生程序中的有用性。Purmorphamine 被用于刺激体外细胞培养测定和体内鸡胚 CAM 测定中的骨生长和修复，同时存在和不存在植入物。Purmorphamine 附着在沉淀的羟基磷灰石涂层上，能够激活 sonic hedgehog 途径，从而刺激成骨分化。多孔磷酸钙珠被用于在体内递送这种小分子，结果表明，Purmorphamine 显著增加了小梁骨与骨区域的比例。该测定表明，Purmorphamine 未能诱导钛涂层 PTFE 植入物上任何明显的骨整合差异。这表明，虽然小分子可以增强成骨作用，并且可能在再生程序中有用，但它未能增强 Ti 涂层植入物的骨整合，这表明这种刺激可能有助于增强由于疾病导致的骨丢失的骨再生，但不能增强植入物的早期稳定性。
