Hałka-Grysińska Aneta, Slązak Piotr, Torbicz Andrzej, Sajewicz Mieczysław, Dzido Tadeusz H
Department of Physical Chemistry, Medical University of Lublin, Chodźki 4a, 20-093 Lublin, Poland.
Chromatographia. 2013;76(19):1271-1279. doi: 10.1007/s10337-013-2430-x. Epub 2013 Mar 5.
Pressurized planar electrochromatography (PPEC) is a separating technique in which an electric field is applied to force the mobile phase movement through a porous media (electroosmotic effect). High separation efficiency, fast separations and changes in separation selectivity in comparison to liquid chromatography, especially thin layer chromatography (planar chromatography, TLC), are features of this technique. Constructional methodological challenges to PPEC are obstacles to its development and application in laboratory practice. In this article, an attempt to overcome the challenges related to device construction and sample application/injection is described. The introduced device enables both prewetting of the adsorbent layer and electrochromatogram development with a single PPEC device. It also enables simultaneous application/injection of six samples on a chromatographic plate in a stream of the mobile phase (on-line application/injection). In addition, the PPEC chamber was equipped with a thermostat. The device is characterized by an impressive throughput in comparison to the other planar technique, TLC/HPTLC. Although the developed device still needs improvement, it is, in our opinion, a considerable step toward possible automation of this planar separation technique.
加压平面电色谱法（PPEC）是一种分离技术，其中施加电场以迫使流动相通过多孔介质移动（电渗效应）。与液相色谱法，尤其是薄层色谱法（平面色谱法，TLC）相比，该技术具有分离效率高、分离速度快以及分离选择性变化等特点。PPEC在构建方法上的挑战是其在实验室实践中发展和应用的障碍。本文描述了克服与设备构建和样品施加/进样相关挑战的尝试。所引入的设备能够使用单个PPEC设备对吸附剂层进行预湿和展开电色谱图。它还能够在流动相流中在色谱板上同时施加/进样六个样品（在线施加/进样）。此外，PPEC室配备了恒温器。与其他平面技术TLC/HPTLC相比，该设备的特点是通量可观。尽管所开发的设备仍需改进，但我们认为，这是朝着这种平面分离技术可能的自动化迈出的重要一步。
