Institute for Clinical Chemistry and Laboratory Medicine, Regensburg University Medical Center, Regensburg, Germany.
Exp Dermatol. 2013 Oct;22(10):677-9. doi: 10.1111/exd.12231.
Keratinocyte sphingolipids are structural elements of epidermal permeability barrier and potential regulators of epidermal functions. We tested the influence of sphingoid bases sphinganine, sphingosine and phytosphingosine on in vitro keratinocyte differentiation. Lipidomic and transcriptomic analysis after treatment emphasizes sphinganine and phytosphingosine as potent modulators of keratinocyte differentiation and lipid metabolism. Sphinganine treatment regulated differentiation and sphingolipid metabolism-related genes, and also increased all major ceramide species. Sphingosine treatment increased ceramide and phytoceramide pools without changes in dihydroceramides. Phytosphingosine treatment markedly increased phytoceramide pools without raising ceramide or dihydroceramide levels. Sphinganine treatment increased specifically very long chain ceramides essential for intact barrier function. In summary, sphingoid bases, especially sphinganine, promote differentiation and ceramide production in keratinocytes. Free sphinganine may serve as a dermatological and cosmetic agent by enhancing formation and maintenance of an intact epidermal lipid barrier, with beneficial effects for skin and hair care applications.
角朊细胞神经酰胺是表皮渗透屏障的结构要素，也是表皮功能的潜在调节剂。我们检测了神经酰胺碱基神经醇、鞘氨醇和植物鞘氨醇对体外角朊细胞分化的影响。处理后的脂质组学和转录组学分析强调了神经醇和植物鞘氨醇是角朊细胞分化和脂质代谢的有效调节剂。神经醇处理调节了分化和神经鞘脂代谢相关基因，还增加了所有主要的神经酰胺种类。鞘氨醇处理增加了神经酰胺和植物神经酰胺的池，但二氢神经酰胺没有变化。植物鞘氨醇处理显著增加了植物神经酰胺池，而没有提高神经酰胺或二氢神经酰胺的水平。神经醇处理特别增加了维持完整屏障功能所必需的非常长链神经酰胺。总之，神经酰胺碱基，特别是神经醇，促进角朊细胞的分化和神经酰胺的产生。游离神经醇可能作为一种皮肤学和化妆品制剂，通过增强完整表皮脂质屏障的形成和维持，对角质层和头发护理应用有有益的效果。
