Department of Veterinary Medicine and Animal Productions, Division of Internal Medicine, University of Naples Federico II, Via Delpino, 1, 80137 Naples, Italy.
Res Vet Sci. 2013 Dec;95(3):942-9. doi: 10.1016/j.rvsc.2013.08.005. Epub 2013 Aug 22.
Canine leishmaniasis caused by the protozoan parasite Leishmania infantum is a chronic systemic disease endemic in Mediterranean basin. The aim of the study is to investigate the immune profile of dogs naturally infected by Leishmania infantum. In order to address such issue, CD4(+) and CD8(+) lymphocyte T cell subsets, peripheral CD4(+)CD3(+)Foxp3(+) (Treg) levels and the presence of pro-inflammatory T cells have been assessed, in 45 infected dogs and in 30 healthy animals, by using immunofluorescence and flow cytometry detection. Animals were categorised according to their clinical-pathological status and their antibody titer at diagnosis. Results showing a significant increase of CD8(+)CD3(+) T lymphocytes, a reduced percentage of the T regulatory CD4(+)CD3(+)Foxp3(+) subset and a significant increase of T(H)1 cells, characterise the infected dogs, regardless of their antibody titer or the occurrence of clinical symptomatic disease. These data may provide new insights into the pathogenesis of immune-mediated alterations associated with canine leishmaniasis.
由原生动物寄生虫利什曼原虫引起的犬利什曼病是一种在地中海盆地流行的慢性全身性疾病。本研究旨在研究自然感染利什曼原虫的犬的免疫特征。为了解决这个问题，通过免疫荧光和流式细胞术检测，在 45 只感染犬和 30 只健康动物中评估了 CD4(+)和 CD8(+)淋巴细胞 T 细胞亚群、外周 CD4(+)CD3(+)Foxp3(+)（Treg）水平和促炎 T 细胞的存在。根据临床病理状态和诊断时的抗体滴度对动物进行分类。结果显示，无论抗体滴度或临床症状疾病的发生如何，感染犬的 CD8(+)CD3(+)T 淋巴细胞显著增加，调节性 CD4(+)CD3(+)Foxp3(+)亚群的百分比降低，T(H)1 细胞显著增加。这些数据可能为犬利什曼病相关免疫介导改变的发病机制提供新的见解。
