Potocnik A J, Kinne R, Menninger H, Zacher J, Emmrich F, Kroczek R A
Max-Planck-Society, Institute for Clinical Immunology, University, Erlangen, FRG.
Scand J Immunol. 1990 Feb;31(2):213-24. doi: 10.1111/j.1365-3083.1990.tb02762.x.
The aim of our study was to identify differences in cell surface marker expression between T cells taken from the peripheral blood (PB) of healthy individuals and T cells recovered from inflamed joints of rheumatoid arthritis (RA) patients. Out of 118 monoclonal antibodies (MoAbs) directed against activation antigens on haematopoietic cells, 12 MoAbs recognizing nine distinct surface molecules were selected after a screening procedure to study the expression of the corresponding antigens on T cells from the PB, synovial fluid and synovial tissue of RA patients, and also on T cells from PB and spleens of controls. Using two-colour flow cytometry and immunohistology we found the molecules B-C5, CD39, CD40, CD45 R0, CD54, CD76 and potentially 1D11 to be substantially up-regulated on T cells from various body compartments in RA patients. We thus could determine that the cell surface of T cells in RA patients not only differs in MHC class II expression, but also in a number of other activation-associated cell surface molecules from T cells in healthy individuals.
我们研究的目的是确定取自健康个体外周血（PB）的T细胞与从类风湿性关节炎（RA）患者发炎关节中分离出的T细胞在细胞表面标志物表达上的差异。在针对造血细胞活化抗原的118种单克隆抗体（MoAbs）中，经过筛选程序后选择了12种识别9种不同表面分子的MoAbs，用于研究相应抗原在RA患者的PB、滑液和滑膜组织中的T细胞上，以及对照的PB和脾脏中的T细胞上的表达情况。使用双色流式细胞术和免疫组织学方法，我们发现分子B-C5、CD39、CD40、CD45 R0、CD54、CD76以及可能的1D11在RA患者不同身体部位的T细胞上显著上调。因此，我们可以确定，RA患者T细胞的细胞表面不仅在MHC II类表达上与健康个体的T细胞不同，而且在许多其他与活化相关的细胞表面分子上也存在差异。
