Afeltra A, Galeazzi M, Ferri G M, Amoroso A, De Pità O, Porzio F, Bonomo L
Institute of Clinical Medicine III, University of Rome, La Sapienza, Italy.
Ann Rheum Dis. 1993 Jun;52(6):457-60. doi: 10.1136/ard.52.6.457.
The expression of the CD69 antigen on synovial fluid and peripheral blood lymphocytes was studied in 12 patients with rheumatoid arthritis (RA), five subjects with other forms of chronic synovitis, and on the peripheral blood lymphocytes of 15 patients with systemic lupus erythematosus (SLE) and immune vasculitis.
The CD69 antigen and other activation markers (HLA-DR, interleukin 2 receptor (IL-2R), transferrin receptor) were measured by cytometric analysis. In patients with RA soluble IL-2R was determined by enzyme linked immunosorbent assay (ELISA).
The percentage of T cells bearing CD69 was significantly increased in synovial fluid from patients with RA (30.3 (13)%) and other chronic synovitis (18 (9)%). The expression of CD69 on peripheral blood lymphocytes of patients with RA, other chronic synovitis, and SLE and immune vasculitis was within the normal range 2.1 (1.2)%. According to previously published work, a high proportion of synovial fluid T cells are HLA-DR positive (64.2 (12.4)% in synovial fluid from patients with RA and 61 (1.2)% in synovial fluid from patients with other chronic synovitis). Transferrin receptor expression on synovial fluid was up-regulated compared with that on peripheral blood. The increase of IL-2R expression on synovial fluid lymphocytes v peripheral blood was not significant; the quantitative determination of soluble IL-2R levels gave a mean value of 921 (351) U/ml in synovial fluid of patients with RA, 672 (229) U/ml in the serum of the same patients, and 273 (100) U/ml in serum from normal subjects.
Synovial fluid lymphocytes are in a different functional state than peripheral blood lymphocytes. CD69 antigen is an interesting cellular marker which should be studied in patients with chronic synovitis. The unusual expression of the activation antigens and the sequence of their appearance require further study.
研究12例类风湿关节炎（RA）患者、5例其他形式慢性滑膜炎患者滑液及外周血淋巴细胞上CD69抗原的表达，并研究15例系统性红斑狼疮（SLE）和免疫性血管炎患者外周血淋巴细胞上CD69抗原的表达。
通过细胞计量分析测定CD69抗原及其他活化标志物（HLA-DR、白细胞介素2受体（IL-2R）、转铁蛋白受体）。对RA患者采用酶联免疫吸附测定（ELISA）法测定可溶性IL-2R。
RA患者（30.3（13）%）和其他慢性滑膜炎患者（18（9）%）滑液中携带CD69的T细胞百分比显著升高。RA患者、其他慢性滑膜炎患者、SLE和免疫性血管炎患者外周血淋巴细胞上CD69的表达在正常范围内，为2.1（1.2）%。根据先前发表的研究，滑液中高比例的T细胞HLA-DR呈阳性（RA患者滑液中为64.2（12.4）%，其他慢性滑膜炎患者滑液中为61（1.2）%）。与外周血相比，滑液中转铁蛋白受体表达上调。滑液淋巴细胞与外周血淋巴细胞相比，IL-2R表达的增加不显著；可溶性IL-2R水平的定量测定结果显示，RA患者滑液中平均值为921（351）U/ml，同一患者血清中为672（229）U/ml，正常受试者血清中为273（100）U/ml。
滑液淋巴细胞与外周血淋巴细胞处于不同的功能状态。CD69抗原是一个有趣的细胞标志物，应在慢性滑膜炎患者中进行研究。活化抗原的异常表达及其出现顺序需要进一步研究。
