Kariatsumari T, Horiuchi M, Hama E, Yaguchi K, Ishigurio N, Goto H, Shinagawa M
Department of Veterinary Public Health, Faculty of Veterinary Medicine, Obihiro University of Agriculture and Veterinary Medicine, Hokkaido, Japan.
J Gen Virol. 1991 Apr;72 ( Pt 4):867-75. doi: 10.1099/0022-1317-72-4-867.
We have cloned the replicative form (RF-) DNA of mink enteritis virus (MEV), constructed an infectious recombinant plasmid containing MEV DNA and determined the nucleotide sequence of the cloned MEV DNA. RF-DNAs were detected and infectious virus was generated when the recombinant plasmid containing the entire MEV genome was introduced into feline kidney cell cultures. The MEV genome was 5094 nucleotides (nt) in length; the 3' end of the virion strand contained a 205 nt palindromic sequence and the 5' end a 62 nt palindromic sequence that could assume Y- and U-shaped configurations, respectively. The 5' end of the virion strand had a direct repeat of 61 nt at the carboxyl terminus of the capsid protein gene. The organization of the MEV genome is similar to those of canine parvovirus (CPV) and feline panleukopenia virus (FPLV); there are two large open reading frames (ORFs), one in the 3' half and the other in the 5' half of the genome, with coding capacities of 668 and 722 amino acid residues, respectively. Both are in the same reading frame and no significant ORFs are apparent in the virion strand (negative-sense strand). Possible functional promoter motifs are located at map unit (m.u.) 4.5 and m.u. 40, and a possible functional poly(A) signal is located at m.u. 96. The nucleotide and amino acid sequence homology with CPV and FPLV is greater than 98%, consistent with the hypothesis that MEV and CPV are host-range variants of FPLV.
我们克隆了水貂肠炎病毒（MEV）的复制型（RF-）DNA，构建了包含MEV DNA的感染性重组质粒，并测定了克隆的MEV DNA的核苷酸序列。当将包含整个MEV基因组的重组质粒引入猫肾细胞培养物中时，检测到了RF-DNAs并产生了感染性病毒。MEV基因组长度为5094个核苷酸（nt）；病毒链的3'端包含一个205 nt的回文序列，5'端包含一个62 nt的回文序列，它们可分别呈现Y形和U形结构。病毒链的5'端在衣壳蛋白基因的羧基末端有一个61 nt的直接重复序列。MEV基因组的组织方式与犬细小病毒（CPV）和猫泛白细胞减少症病毒（FPLV）相似；有两个大的开放阅读框（ORF），一个在基因组的3'半部分，另一个在5'半部分，编码能力分别为668和722个氨基酸残基。两者处于相同的阅读框，在病毒链（负义链）中没有明显的重要ORF。可能的功能性启动子基序位于图距单位（m.u.）4.5和m.u. 40处，一个可能的功能性聚腺苷酸化（poly(A)）信号位于m.u. 96处。与CPV和FPLV的核苷酸和氨基酸序列同源性大于98%，这与MEV和CPV是FPLV的宿主范围变体这一假设一致。
