Division of Genetics and Molecular Biology, Institute of Biological Sciences, Faculty of Science, University of Malaya, Kuala Lumpur 50603, Malaysia.
Sensors (Basel). 2013 Sep 30;13(10):13192-203. doi: 10.3390/s131013192.
Bacterial cell-to-cell communication (quorum sensing) refers to the regulation of bacterial gene expression in response to changes in microbial population density. Quorum sensing bacteria produce, release and respond to chemical signal molecules called autoinducers. Bacteria use two types of autoinducers, namely autoinducer-1 (AI-1) and autoinducer-2 (AI-2) where the former are N-acylhomoserine lactones and the latter is a product of the luxS gene. Most of the reported literatures show that the majority of oral bacteria use AI-2 for quorum sensing but rarely the AI-1 system. Here we report the isolation of Pseudomonas putida strain T2-2 from the oral cavity. Using high resolution mass spectrometry, it is shown that this isolate produced N-octanoylhomoserine lactone (C8-HSL) and N-dodecanoylhomoserine lactone (C12-HSL) molecules. This is the first report of the finding of quorum sensing of P. putida strain T2-2 isolated from the human tongue surface and their quorum sensing molecules were identified.
细菌细胞间通讯（群体感应）是指细菌根据微生物种群密度的变化来调节基因表达。群体感应细菌产生、释放并响应被称为自诱导物的化学信号分子。细菌使用两种类型的自诱导物，即自诱导物-1（AI-1）和自诱导物-2（AI-2），前者是 N-酰基高丝氨酸内酯，后者是 luxS 基因的产物。大多数报道的文献表明，大多数口腔细菌使用 AI-2 进行群体感应，但很少使用 AI-1 系统。在这里，我们报告了从口腔中分离出假单胞菌 T2-2 菌株。使用高分辨率质谱，表明该分离物产生 N-辛酰基高丝氨酸内酯（C8-HSL）和 N-十二酰基高丝氨酸内酯（C12-HSL）分子。这是首次报道从人舌表面分离出的假单胞菌 T2-2 菌株的群体感应及其群体感应分子的鉴定。
