Division of Genetics and Molecular Biology, Institute of Biological Sciences, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, Malaysia.
Sensors (Basel). 2012 Oct 24;12(11):14307-14. doi: 10.3390/s121114307.
We report the isolation of N-acyl homoserine lactone-producing Enterobacter sp. isolate T1-1 from the posterior dorsal surfaces of the tongue of a healthy individual. Spent supernatants extract from Enterobacter sp. isolate T1-1 activated the biosensor Agrobacterium tumefaciens NTL4(pZLR4), suggesting production of long chain AHLs by these isolates. High resolution mass spectrometry analysis of these extracts confirmed that Enterobacter sp. isolate T1-1 produced a long chain N-acyl homoserine lactone, namely N-dodecanoyl-homoserine lactone (C12-HSL). To the best of our knowledge, this is the first isolation of Enterobacter sp., strain T1-1 from the posterior dorsal surface of the human tongue and N-acyl homoserine lactones production by this bacterium.
我们从一位健康个体的舌背后部表面分离到产生 N-酰基高丝氨酸内酯的肠杆菌属 sp. 分离株 T1-1。肠杆菌属 sp. 分离株 T1-1 的废弃上清液提取物激活了生物传感器根癌农杆菌 NTL4(pZLR4)，表明这些分离株产生了长链 AHLs。这些提取物的高分辨质谱分析证实肠杆菌属 sp. 分离株 T1-1 产生了长链 N-酰基高丝氨酸内酯，即 N-十二烷酰基高丝氨酸内酯 (C12-HSL)。据我们所知，这是首次从人舌背后部表面分离到肠杆菌属 sp. 菌株 T1-1，并且该细菌能够产生 N-酰基高丝氨酸内酯。
