Zajdel J K, Ceglowski P, Dobrazański W T
Appl Environ Microbiol. 1985 Apr;49(4):969-74. doi: 10.1128/aem.49.4.969-974.1985.
The mechanism of bactericidal activity of lactostrepcin 5 (Las 5), a bacteriocin produced by Streptococcus cremoris 202, was investigated. Las 5 did not kill protoplasts of sensitive cells, and its activity was decreased about 10-fold after pretreatment of the cells with trypsin, suggesting the involvement of the cell wall in the activity of this bacteriocin. In susceptible cells, the bacteriocin slowed down and then stopped synthesis of DNA, RNA, and protein, although this did not appear to be the primary effect of Las 5 action. Las 5 also inhibited uridine transport in susceptible cells and induced leakage of K+ ions and ATP. Survival of cells treated with Las 5 in phosphate buffer was higher in the presence of K+, CA2+, or Mg2+ ions.
研究了由嗜热链球菌202产生的一种细菌素——乳链菌肽5（Las 5）的杀菌活性机制。Las 5不能杀死敏感细胞的原生质体，并且在用胰蛋白酶预处理细胞后其活性降低了约10倍，这表明细胞壁参与了这种细菌素的活性。在敏感细胞中，细菌素减缓并随后停止DNA、RNA和蛋白质的合成，尽管这似乎不是Las 5作用的主要效应。Las 5还抑制敏感细胞中的尿苷转运，并诱导K+离子和ATP的泄漏。在磷酸盐缓冲液中用Las 5处理的细胞在存在K+、Ca2+或Mg2+离子的情况下存活率更高。
