Sahl H G, Brandis H
Zentralbl Bakteriol Mikrobiol Hyg A Med Mikrobiol Infekt Parasitol. 1982 Jun;252(2):166-75.
Pep 5, a staphylococcin-like peptide produced by Staphylococcus epidermidis 5 was bactericidal for staphylococci and micrococci (Fig. 1). A stable L-phase variant of Staphylococcus aureus, but not of Streptococcus faecalis, was susceptible, too, indicating that the selectivity of Pep 5 is not due to the presence of a cell wall receptor. Addition of Pep 5 to cultures of the indicator strain caused immediate inhibition of DNA, RNA, protein, and polysaccharide synthesis (Fig. 5). The action of Pep 5 could be inhibited by increasing the cation content of the medium (e.g., to 100 mM Mg2+ or 600 mM K+) (Fig. 3), indicating a primary binding to anionic sites on the cell wall. Sublethally injured cells could be rescued by the addition of trypsin or 1 M KCl (Fig. 4).
表皮葡萄球菌5产生的类葡萄球菌素肽Pep 5对葡萄球菌和微球菌具有杀菌作用（图1）。金黄色葡萄球菌的稳定L型变体对其敏感，但粪肠球菌的稳定L型变体不敏感，这表明Pep 5的选择性并非由于细胞壁受体的存在。向指示菌株的培养物中添加Pep 5会立即抑制DNA、RNA、蛋白质和多糖的合成（图5）。通过增加培养基中的阳离子含量（例如，至100 mM Mg2+或600 mM K+）可抑制Pep 5的作用（图3），这表明它首先与细胞壁上的阴离子位点结合。添加胰蛋白酶或1 M KCl可挽救受到亚致死损伤的细胞（图4）。
