Enberg G
Biochem Biophys Res Commun. 1986 Feb 26;135(1):178-82. doi: 10.1016/0006-291x(86)90959-9.
The somatomedins insulin-like growth factor I and II (1,2) are in serum bound to high-molecular weight binding proteins (6,7,8). By use of a four step chromatographic procedure a somatomedin binding protein was isolated from outdated human plasma. Exclusion chromatography on Sephadex G-200 disclosed a molecular weight of 150 kDa. After lyophilization however, the binding activity was found in a lower molecular weight range of 35-45 kDa. A partial amino acid sequence analysis of the lyophilized material revealed a possible N-terminal sequence of Ala-Pro-Trp. This sequence is identical to the N-terminal sequence of the 35 kDa somatomedin binding protein previously isolated from human amniotic fluid (16).
生长调节素胰岛素样生长因子I和II（1,2）在血清中与高分子量结合蛋白结合（6,7,8）。通过四步色谱法从过期人血浆中分离出一种生长调节素结合蛋白。在葡聚糖凝胶G - 200上进行排阻色谱显示分子量为150 kDa。然而，冻干后，结合活性出现在35 - 45 kDa的较低分子量范围内。对冻干材料的部分氨基酸序列分析揭示了可能的N端序列为丙氨酸 - 脯氨酸 - 色氨酸。该序列与先前从人羊水分离出的35 kDa生长调节素结合蛋白的N端序列相同（16）。
