Ezquerra A, Bragado R, Vega M A, Strominger J L, Woody J, López de Castro J A
Biochemistry. 1985 Mar 26;24(7):1733-41. doi: 10.1021/bi00328a025.
The complete amino acid sequence of papain-solubilized HLA-B27, an antigen that presents a very strong association to the development of ankylosing spondylitis, has been determined. The overall sequence homology with the cross-reactive allelic products HLA-B7 and HLA-B40 (Bw60) is 93% and 92%, respectively. Half of the differences between HLA-B27 and -B7 are located in segments 63-83 and 113-116. Most of the known HLA class I antigens are different in these segments, and it is suggested that the corresponding residues may be involved in the alloantigenic determinants of HLA-B27. A free cysteine residue is present at position 67, and it is at least partially exposed to solvent. In addition, other differences are found in various areas of the two N-terminal domains. The comparison with available HLA class I sequences allows an evaluation of their contribution to the antigenic polymorphism of these molecules. The relevance of these data is discussed in connection with the mapping of functional sites of HLA class I antigens and with the association between HLA-B27 and ankylosing spondylitis.
木瓜蛋白酶可溶解的HLA - B27的完整氨基酸序列已被确定，HLA - B27是一种与强直性脊柱炎的发展呈现出非常强关联的抗原。与交叉反应等位基因产物HLA - B7和HLA - B40（Bw60）的总体序列同源性分别为93%和92%。HLA - B27和 - B7之间一半的差异位于63 - 83段和113 - 116段。大多数已知的HLA I类抗原在这些片段中是不同的，并且有人提出相应的残基可能参与了HLA - B27的同种异体抗原决定簇。在第67位存在一个游离的半胱氨酸残基，并且它至少部分暴露于溶剂中。此外，在两个N端结构域的各个区域还发现了其他差异。与现有的HLA I类序列进行比较，可以评估它们对这些分子抗原多态性的贡献。结合HLA I类抗原功能位点的定位以及HLA - B27与强直性脊柱炎之间的关联，对这些数据的相关性进行了讨论。
