Zhang Yangyang, Shi Wen, Li Xiaohua, Ma Huimin
Beijing National Laboratory for Molecular Sciences, Key Laboratory of Analytical Chemistry for Living Biosystems, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100190, China.
Sci Rep. 2013 Oct 3;3:2830. doi: 10.1038/srep02830.
Ozone (O3) has attracted much attention because of its key role in human health and disease, and its detection is of great importance for various biochemical studies as well as environmental evaluation. Here we develop a simple and practical spectroscopic off-on O3 probe based on resorufin and the specific reaction of but-3-enyl with O3. The probe shows an extremely low background spectroscopic signal, but reacts with O3 producing a distinct color and fluorescence change. The detection limit of the probe for O3 is 5.9 nM, which corresponds to an ozone concentration of 0.056 mg m(-3) in air in this study and is lower than the international ambient air quality standard of 0.1 mg m(-3). More importantly, the proposed probe is worth popularizing, and its applicability has been successfully demonstrated on both the determination of O3 in real ambient air samples and the imaging of O3 in biological cells.
臭氧（O₃）因其在人类健康与疾病中的关键作用而备受关注，其检测对于各种生化研究以及环境评估都具有重要意义。在此，我们基于试卤灵以及3-丁烯基与O₃的特定反应，开发了一种简单实用的光谱开-关型O₃探针。该探针显示出极低的背景光谱信号，但与O₃反应时会产生明显的颜色和荧光变化。本研究中该探针对O₃的检测限为5.9 nM，这相当于空气中臭氧浓度为0.056 mg m⁻³，低于国际环境空气质量标准0.1 mg m⁻³。更重要的是，所提出的探针值得推广，并且已成功证明其在实际环境空气样品中O₃测定以及生物细胞中O₃成像方面的适用性。
