Radboud University Nijmegen, Life Science Trace Gas Research Group, Molecular and Laser Physics, Institute for Molecules and Materials, Nijmegen, The Netherlands.
J Biomed Opt. 2013 Oct;18(10):107002. doi: 10.1117/1.JBO.18.10.107002.
A versatile, continuous wave, optical parametric oscillator is used in combination with photoacoustic spectroscopy for long-term trace gas experiments of volatile compounds emitted by biological samples. The optical parametric oscillator-based spectrometer (wavelength near 3 μm, 8-MHz linewidth, output power ∼1 W) is successfully tested for the detection of hydrogen cyanide (HCN) emission from clover leaves, and Pseudomonas bacteria; in addition, the presence of HCN in exhaled human breath is measured. For specific experiments, the spectrometer is operated continuously up to 10 days and has a detection limit of 0.4 parts-per-billion volume of HCN in air over 10 s, using the P8 rotational line in the ν₃ vibrational band of HCN at 3287.25 cm⁻¹. This results in an overall sensitivity of the system of 2.5 × 10⁻⁹ cm-1 Hz⁻¹/².
一种多功能、连续波的光参量振荡器与光声光谱学结合，用于生物样品挥发物痕量气体的长期实验。基于光参量振荡器的光谱仪（波长接近 3μm，8MHz 线宽，输出功率约 1W）成功地用于检测三叶草叶片和假单胞菌的氢氰酸（HCN）排放；此外，还测量了人呼气中 HCN 的存在。对于特定实验，光谱仪连续运行长达 10 天，使用 HCN 的 ν₃振动带中的 P8 转动线，在 3287.25cm⁻¹处，在 10s 内空气中 HCN 的检测限为 0.4 部分-每十亿分之 HCN，这导致系统的整体灵敏度为 2.5×10⁻⁹cm-1Hz⁻¹/²。
