基于光学参量振荡器的生物医学应用中氰化氢光声检测。

Optical parametric oscillator-based photoacoustic detection of hydrogen cyanide for biomedical applications.

机构信息

Radboud University Nijmegen, Life Science Trace Gas Research Group, Molecular and Laser Physics, Institute for Molecules and Materials, Nijmegen, The Netherlands.

出版信息

J Biomed Opt. 2013 Oct;18(10):107002. doi: 10.1117/1.JBO.18.10.107002.

DOI:10.1117/1.JBO.18.10.107002

PMID:24089255

Abstract

A versatile, continuous wave, optical parametric oscillator is used in combination with photoacoustic spectroscopy for long-term trace gas experiments of volatile compounds emitted by biological samples. The optical parametric oscillator-based spectrometer (wavelength near 3 μm, 8-MHz linewidth, output power ∼1 W) is successfully tested for the detection of hydrogen cyanide (HCN) emission from clover leaves, and Pseudomonas bacteria; in addition, the presence of HCN in exhaled human breath is measured. For specific experiments, the spectrometer is operated continuously up to 10 days and has a detection limit of 0.4 parts-per-billion volume of HCN in air over 10 s, using the P8 rotational line in the ν₃ vibrational band of HCN at 3287.25 cm⁻¹. This results in an overall sensitivity of the system of 2.5 × 10⁻⁹ cm-1 Hz⁻¹/².

摘要

一种多功能、连续波的光参量振荡器与光声光谱学结合，用于生物样品挥发物痕量气体的长期实验。基于光参量振荡器的光谱仪（波长接近 3μm，8MHz 线宽，输出功率约 1W）成功地用于检测三叶草叶片和假单胞菌的氢氰酸（HCN）排放；此外，还测量了人呼气中 HCN 的存在。对于特定实验，光谱仪连续运行长达 10 天，使用 HCN 的 ν₃振动带中的 P8 转动线，在 3287.25cm⁻¹处，在 10s 内空气中 HCN 的检测限为 0.4 部分-每十亿分之 HCN，这导致系统的整体灵敏度为 2.5×10⁻⁹cm-1Hz⁻¹/²。