Henderson Ben, Khodabakhsh Amir, Metsälä Markus, Ventrillard Irène, Schmidt Florian M, Romanini Daniele, Ritchie Grant A D, Te Lintel Hekkert Sacco, Briot Raphaël, Risby Terence, Marczin Nandor, Harren Frans J M, Cristescu Simona M
1Trace Gas Research Group, Molecular and Laser Physics, IMM, Radboud University, 6525 AJ Nijmegen, The Netherlands.
2Department of Chemistry, University of Helsinki, PO Box 55, 00014 Helsinki, Finland.
Appl Phys B. 2018;124(8):161. doi: 10.1007/s00340-018-7030-x. Epub 2018 Jul 28.
Detection and analysis of volatile compounds in exhaled breath represents an attractive tool for monitoring the metabolic status of a patient and disease diagnosis, since it is non-invasive and fast. Numerous studies have already demonstrated the benefit of breath analysis in clinical settings/applications and encouraged multidisciplinary research to reveal new insights regarding the origins, pathways, and pathophysiological roles of breath components. Many breath analysis methods are currently available to help explore these directions, ranging from mass spectrometry to laser-based spectroscopy and sensor arrays. This review presents an update of the current status of optical methods, using near and mid-infrared sources, for clinical breath gas analysis over the last decade and describes recent technological developments and their applications. The review includes: tunable diode laser absorption spectroscopy, cavity ring-down spectroscopy, integrated cavity output spectroscopy, cavity-enhanced absorption spectroscopy, photoacoustic spectroscopy, quartz-enhanced photoacoustic spectroscopy, and optical frequency comb spectroscopy. A SWOT analysis (strengths, weaknesses, opportunities, and threats) is presented that describes the laser-based techniques within the clinical framework of breath research and their appealing features for clinical use.
呼出气体中挥发性化合物的检测与分析是监测患者代谢状态和疾病诊断的一种有吸引力的工具，因为它具有非侵入性且快速的特点。众多研究已经证明了呼气分析在临床环境/应用中的益处，并鼓励多学科研究以揭示有关呼气成分的来源、途径和病理生理作用的新见解。目前有许多呼气分析方法可用于探索这些方向，从质谱分析到基于激光的光谱分析和传感器阵列。本综述介绍了过去十年中使用近红外和中红外光源的光学方法在临床呼气气体分析方面的最新进展，并描述了最近的技术发展及其应用。该综述包括：可调谐二极管激光吸收光谱、光腔衰荡光谱、集成腔输出光谱、腔增强吸收光谱、光声光谱、石英增强光声光谱和光学频率梳光谱。本文进行了SWOT分析（优势、劣势、机会和威胁），描述了基于激光的技术在呼气研究临床框架内的情况及其在临床应用中的吸引人的特点。
