Laboratory of Physical Chemistry, Department of Chemistry, PO Box 55 (A.I. Virtasen aukio 1), FIN-00014 University of Helsinki, Finland.
J Breath Res. 2011 Dec;5(4):046004. doi: 10.1088/1752-7155/5/4/046004. Epub 2011 Aug 2.
The hydrogen cyanide (HCN) concentration in exhaled human breath and skin gas samples collected with different sampling techniques was measured using near-infrared cavity ring-down spectroscopy. The median baseline HCN concentrations in samples provided by 19 healthy volunteers 2-4 h after the last meal depended on the employed sampling technique: 6.5 parts per billion by volume (ppbv) in mixed (dead space and end-tidal) mouth-exhaled breath collected to a gas sampling bag, 3.9 ppbv in end-tidal mouth-exhaled breath, 1.3 ppbv in end-tidal nose-exhaled breath, 1.0 ppbv in unwashed skin and 0.6 ppbv in washed skin samples. Diurnal measurements showed that elevated HCN levels are to be expected in mouth-exhaled breath samples after food and drink intake, which suggests HCN generation in the oral cavity. The HCN concentrations in end-tidal nose-exhaled breath and skin gas samples were correlated, and it is concluded that these concentrations best reflect systemic HCN levels.
采用近红外光腔衰荡光谱法测量了不同采样技术采集的呼出人体呼吸和皮肤气体样本中的氰化氢（HCN）浓度。19 名健康志愿者在最后一餐 2-4 小时后提供的样本中，HCN 的基线中位数浓度取决于所采用的采样技术：混合（死腔和呼气末）口腔呼出呼吸到气体采样袋中的体积浓度为 6.5 十亿分之一（ppbv），呼气末口腔呼出呼吸中的浓度为 3.9 ppbv，呼气末鼻腔呼出呼吸中的浓度为 1.3 ppbv，未清洗皮肤中的浓度为 1.0 ppbv，清洗皮肤样本中的浓度为 0.6 ppbv。日间测量表明，在摄入食物和饮料后，口腔呼出呼吸样本中的 HCN 水平会升高，这表明口腔中 HCN 的生成。呼气末鼻腔呼出呼吸和皮肤气体样本中的 HCN 浓度相关，因此可以得出结论，这些浓度最好反映了系统中的 HCN 水平。
