Cox D S, Furman S, Muench D
Immunol Invest. 1985 Apr;14(2):151-9. doi: 10.3109/08820138509042009.
Sprague-Dawley rats were administered DNP-BGG on day one of pregnancy by either local mammary gland vicinity injection or by gastric intubation. All rats later received local mammary gland vicinity injection of DNP-BGG on day 11 of pregnancy. Milk and serum were collected post-parturition. IgA and IgG antibody was separated in the milk and IgG antibody in the serum. The relative average affinity constant (KO) was determined by a modification of the Farr assay. Milk IgG affinity was found to be considerably higher than the affinity of IgG in the serum. IgA affinity in the milk of rats which received the antigen by gastric intubation demonstrated antibody affinity much greater than the affinity of milk antibody in rats which simply received mammary gland vicinity injection (1.8 x 10(8) vs. 1.4 x 10(6) l/M). The results indicate that much of the IgG antibody in milk may be locally synthesized. Gut-associated lymphoid tissue may act as a source of antigen specific memory cells for peripheral secretory tissues.
在怀孕第一天，通过局部乳腺附近注射或胃插管的方式给斯普拉格-道利大鼠施用二硝基苯-牛血清白蛋白（DNP-BGG）。所有大鼠在怀孕第11天均接受局部乳腺附近注射DNP-BGG。产后收集乳汁和血清。从乳汁中分离出IgA和IgG抗体，从血清中分离出IgG抗体。相对平均亲和常数（KO）通过对Farr测定法的改进来确定。发现乳汁中IgG的亲和力明显高于血清中IgG的亲和力。通过胃插管接受抗原的大鼠乳汁中的IgA亲和力显示出比仅接受乳腺附近注射的大鼠乳汁抗体亲和力大得多（1.8×10⁸对1.4×10⁶ l/M）。结果表明，乳汁中的许多IgG抗体可能是局部合成的。肠道相关淋巴组织可能作为外周分泌组织抗原特异性记忆细胞的来源。
