Metgud Rashmi, Gupta Kanupriya
Department of Oral and Maxillofacial Pathology, Pacific Dental College and Hospital, PAHER University, Udaipur, Rajasthan, India.
Ann Diagn Pathol. 2013 Dec;17(6):518-21. doi: 10.1016/j.anndiagpath.2013.06.006. Epub 2013 Oct 1.
Tumors arising from epithelium of the odontogenic apparatus or from its derivatives or remnants exhibit considerable histologic variation and are classified into several benign and malignant entities. A high proliferative activity of the odontogenic epithelium in ameloblastoma (AM) and keratocystic odontogenic tumor (KCOT) has been demonstrated in some studies individually. However, very few previous studies have simultaneously evaluated cell proliferation and apoptotic indexes in AM and KCOT, comparing both lesions. The aim of this study was to assess and compare cell proliferation and apoptotic rates between these two tumors. Specimens of 15 solid AM and 15 KCOT were evaluated. The proliferation index (PI) was assessed by immunohistochemical detection of Ki-67 and the apoptotic index (AI) by methyl green-pyronin stain. KCOT presented a higher PI than AM (P < .05). No statistically significant difference was found in the AI between AM and KCOT. PI and AI were higher in the peripheral cells of AM and respectively in the suprabasal and superficial layers of KCOT. In conclusion, KCOT showed a higher cell proliferation than AM and the AI was similar between these tumors. These findings reinforce the classification of KCOT as an odontogenic tumor and should contribute to its aggressive clinical behavior.
源自牙源性器官上皮或其衍生物或残余物的肿瘤表现出相当大的组织学差异，可分为几种良性和恶性实体。一些研究分别证实了成釉细胞瘤（AM）和牙源性角化囊性瘤（KCOT）中牙源性上皮的高增殖活性。然而，以往很少有研究同时评估AM和KCOT中的细胞增殖和凋亡指数，并对这两种病变进行比较。本研究的目的是评估和比较这两种肿瘤之间的细胞增殖和凋亡率。对15例实性AM和15例KCOT的标本进行了评估。通过免疫组化检测Ki-67评估增殖指数（PI），通过甲基绿-派洛宁染色评估凋亡指数（AI）。KCOT的PI高于AM（P < 0.05）。AM和KCOT之间的AI未发现统计学上的显著差异。AM的外周细胞以及KCOT的基底上层和表层的PI和AI较高。总之，KCOT的细胞增殖高于AM，且这两种肿瘤的AI相似。这些发现强化了KCOT作为牙源性肿瘤的分类，并应有助于解释其侵袭性的临床行为。
