Departamento de Ciencias Clínicas Veterinarias, Facultad de Veterinaria, Universidad de Santiago de Compostela, 27002 Lugo, Spain.
Microb Pathog. 2013 Dec;65:82-8. doi: 10.1016/j.micpath.2013.09.003. Epub 2013 Oct 1.
Tenacibaculum maritimum is the causative agent of tenacibaculosis, a bacterial disease with a worldwide distribution, which causes important losses in the turbot aquaculture. Despite the importance of this bacterium, little is known about pathogenesis of the tenacibaculosis, pattern of lesions and the portal of entry of T. maritimum. Turbots (Psetta maxima) were experimentally infected with T. maritimum using subcutaneous and intraperitoneal routes of inoculation and samples of skin and internal organs were taken throughout the assay. Fish inoculated by both infection routes suffered a septicaemia but only the subcutaneous inoculation reproduces the disease signs described in natural outbreaks. Bacterial antigen was detected by immunohistochemistry in the internal organs 3 h after infection in fish inoculated by subcutaneous route and 6 h after the inoculation of fish by intraperitoneal route. In summary, both routes of inoculation are able to cause an infection and bacteraemia in the fish. However, subcutaneous inoculation route reproduces the disease in a faster and more reliable way than the intraperitoneal route. Moreover, bacterium spreads along the internal organs easily, but needs a gateway to penetrate in the organism and this portal of entry could be skin.
海洋弯曲杆菌是海洋弯曲菌病的病原体，这是一种具有世界分布的细菌性疾病，给大菱鲆养殖业造成了重大损失。尽管这种细菌很重要，但对海洋弯曲菌病的发病机制、病变模式和海洋弯曲杆菌的入口知之甚少。使用皮下和腹腔接种途径，用海洋弯曲杆菌对大菱鲆进行了实验感染，并在整个试验过程中采集了皮肤和内脏器官的样本。通过这两种感染途径接种的鱼类都发生了败血症，但只有皮下接种才能复制自然爆发中描述的疾病症状。通过免疫组织化学在皮下接种感染后 3 小时和腹腔接种感染后 6 小时的鱼的内脏器官中检测到细菌抗原。总之，这两种接种途径都能引起鱼类感染和菌血症。然而，与腹腔接种途径相比，皮下接种途径以更快、更可靠的方式复制疾病。此外，细菌很容易沿着内脏器官传播，但需要一个进入生物体的门户，这个入口可能是皮肤。
