Faílde Luis Daniel, Losada Ana Paula, Bermúdez Roberto, Santos Ysabel, Quiroga M Isabel
Departamento de Ciencias Clínicas Veterinarias, Facultad de Veterinaria, Universidad de Santiago de Compostela, 27002 Lugo, Spain.
Departamento de Ciencias Clínicas Veterinarias, Facultad de Veterinaria, Universidad de Santiago de Compostela, 27002 Lugo, Spain.
Microb Pathog. 2014 Nov;76:1-9. doi: 10.1016/j.micpath.2014.08.008. Epub 2014 Sep 16.
Tenacibaculum maritimum is a Gram-negative bacterium that causes large losses in farmed turbot, Psetta maxima (L.). Main lesions included erosive and ulcerative skin injuries in different locations in the body of the fish. This study was set up to gain insights into the immune response of the turbot against this bacterial infection. To determine the variations in the peripheral blood leukocytes a minimum of 200 leukocytes in blood smears were counted. The presence of immunoglobulin positive (Ig(+)) cells in spleen, kidney, intestine, thymus and skin from turbot experimentally infected with T. maritimum were studied using an immunohistochemical method. In fish challenged with the bacterium the percentage of circulating granulocytes, lymphocytes and trombocytes showed significant differences at different points of sampling. Moreover, the number of Ig(+) cells significantly increased in the spleen, kidney, intestine and thymus. In the skin a strong immunoreactivity was observed in the interstitial liquid in damaged areas. The results obtained in this study indicated that the infection by T. maritimum in turbot induced an immune response that involved changes in the number of peripheral blood leukocytes and in the number and distribution of Ig(+) cells.
海生黄杆菌是一种革兰氏阴性菌，可导致养殖大菱鲆（Psetta maxima (L.)）遭受巨大损失。主要病变包括鱼体不同部位的糜烂性和溃疡性皮肤损伤。本研究旨在深入了解大菱鲆针对这种细菌感染的免疫反应。为了确定外周血白细胞的变化，对血涂片上至少200个白细胞进行了计数。采用免疫组织化学方法研究了实验感染海生黄杆菌的大菱鲆脾脏、肾脏、肠道、胸腺和皮肤中免疫球蛋白阳性（Ig(+)）细胞的存在情况。在用该细菌攻击的鱼中，循环粒细胞、淋巴细胞和血小板的百分比在不同采样点显示出显著差异。此外，脾脏、肾脏、肠道和胸腺中Ig(+)细胞的数量显著增加。在皮肤中，在受损区域的组织液中观察到强烈的免疫反应性。本研究获得的结果表明，海生黄杆菌感染大菱鲆诱导了一种免疫反应，该反应涉及外周血白细胞数量以及Ig(+)细胞数量和分布的变化。
