Ottenhoff T H, Elferink D G, Termijtelen A, Koning F, de Vries R R
Hum Immunol. 1985 Jun;13(2):117-23. doi: 10.1016/0198-8859(85)90018-7.
We have studied the HLA class II restriction repertoire of antigen-specific T lymphoblasts (T-LB) in response to purified protein derivative (PPD) and tetanus toxoid (TET), presented by allogeneic antigen-presenting cells (APC). In 102 fully DR(1-w14) mismatched T-LB/APC combinations matching for DRw53 (MT3) had a significant influence on T-LB proliferation (p = 0.0005). Moreover, the supertypic specificity DRw52 (MT2) and LB-Q1 (a new class II determinant in strong linkage disequilibrium with DRw52) appeared to be markers for a new RD (p less than 0.0005). LB-Q1 was most strongly associated with this RD and among DRw52 identical T-LB/APC combinations additional LB-Q1 sharing significantly increased T-LB responsiveness (p = 0.02). DRw52- and LB-Q1-restricted responses could be inhibited by an anti-DRw52 and an anti-DR framework monoclonal antibody, indicating that DR(w52), LB-Q1, and the new RD are located at the same molecule.
我们研究了同种异体抗原呈递细胞（APC）呈递的纯化蛋白衍生物（PPD）和破伤风类毒素（TET）刺激下，抗原特异性T淋巴母细胞（T-LB）的HLA II类限制库。在102个完全DR（1-w14）不匹配的T-LB/APC组合中，与DRw53（MT3）匹配对T-LB增殖有显著影响（p = 0.0005）。此外，超型特异性DRw52（MT2）和LB-Q1（与DRw52处于强连锁不平衡的一种新的II类决定簇）似乎是一种新的RD的标志物（p小于0.0005）。LB-Q1与这种RD关联最为紧密，在DRw52相同的T-LB/APC组合中，额外的LB-Q1共享显著增加了T-LB反应性（p = 0.02）。DRw52和LB-Q1限制的反应可被抗DRw52和抗DR框架单克隆抗体抑制，表明DR（w52）、LB-Q1和新的RD位于同一分子上。
