对DNA介导的共转化后在小鼠细胞系中表达的单个HLA DR-α和DR-β链基因产物进行血清学和免疫化学分析，结果显示β链具有一种已知的超型特异性。
Serological and immunochemical analysis of the products of a single HLA DR-alpha and DR-beta chain gene expressed in a mouse cell line after DNA-mediated cotransformation reveals that the beta chain carries a known supertypic specificity.
作者信息
Gorski J, Tosi R, Strubin M, Rabourdin-Combe C, Mach B
出版信息
J Exp Med. 1985 Jul 1;162(1):105-16. doi: 10.1084/jem.162.1.105.
Abstract
Using a mouse cell line transformed with and expressing a single HLA DR-alpha and DR-beta chain gene, we present evidence that the product of the DR-beta chain gene carries a supertypic determinant, BR3, previously defined by serology. The amino acid sequence of this beta chain gene is determined from the DNA sequence. Another DR-associated supertypic specificity defined by monoclonal antibody MCS7 was not encoded by this DR-beta chain gene. This provides formal proof that a supertypic specificity can be associated with a product of a distinct DR-beta locus. We propose that haplotypes sharing such specificities are evolutionarily related.
摘要
利用一种经转化并表达单个HLA DR-α和DR-β链基因的小鼠细胞系，我们提供了证据表明DR-β链基因的产物携带一种超型决定簇BR3，该决定簇先前已通过血清学定义。此β链基因的氨基酸序列由DNA序列确定。由单克隆抗体MCS7定义的另一种与DR相关的超型特异性并非由该DR-β链基因编码。这提供了正式证据，证明一种超型特异性可与一个独特的DR-β基因座的产物相关联。我们提出，共享此类特异性的单倍型在进化上是相关的。
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Serological and immunochemical analysis of the products of a single HLA DR-alpha and DR-beta chain gene expressed in a mouse cell line after DNA-mediated cotransformation reveals that the beta chain carries a known supertypic specificity.对DNA介导的共转化后在小鼠细胞系中表达的单个HLA DR-α和DR-β链基因产物进行血清学和免疫化学分析，结果显示β链具有一种已知的超型特异性。
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