Tosi R, Tanigaki N, Centis D, Rossi P L, Alfano G, Ferrara G B, Pressman D
Transplantation. 1980 Apr;29(4):302-5. doi: 10.1097/00007890-198004000-00008.
A radioimmunoassay procedure is described by which peripheral blood lymphocytes can be typed for HLA-DR specificities. The major advantages of this method are the following: simple and reproducible procedure, no need for B lymphocyte separation, no need for optimal viability, and no need for preabsorption of antisera with platelets. This method will find an application in the genetic and biochemical analysis of the HLA complex, and in the clinical tests of Ia antigens for diagnostic or prognostic purposes and in retrospective transplant studies.
本文描述了一种放射免疫分析方法，通过该方法可对外周血淋巴细胞进行HLA-DR特异性分型。该方法的主要优点如下：操作简单且可重复，无需分离B淋巴细胞，无需最佳活力，也无需用血小板对抗血清进行预吸收。该方法将在HLA复合体的遗传和生化分析、Ia抗原用于诊断或预后目的的临床试验以及回顾性移植研究中得到应用。
