Aikawa R, Ohyama K, Mori E, Hironaka K, Miyake M, Morishita M
J Pharm Sci. 1985 Mar;74(3):300-3. doi: 10.1002/jps.2600740315.
An enzyme immunoassay for the direct measurement of elcatonin, a synthetic analogue of eel calcitonin with hypocalcemic activity, in rat and dog plasma was developed. Elcatonin was conjugated to beta-galactosidase using an N-succinimidyl ester, and the product was used as the tracer. The antibody-bound tracer was precipitated using a second antibody. The nonequilibrated procedure gave better sensitivity and precision than the equilibrated procedure. The nonequilibrated method was sensitive at quantifiable concentrations as low as 0.2 ng/mL using 0.1 mL of plasma and was also reproducible, with RSD values generally less than +/- 10%. Plasma levels in rats and dogs were determined by this competitive enzyme immunoassay after intramuscular or intravenous injection of 40 U/kg (8 micrograms/kg) of elcatonin.
开发了一种酶免疫分析法，用于直接测定大鼠和犬血浆中的依降钙素（鳗鱼降钙素的合成类似物，具有降钙活性）。使用N-琥珀酰亚胺酯将依降钙素与β-半乳糖苷酶偶联，产物用作示踪剂。使用第二抗体沉淀与抗体结合的示踪剂。非平衡法比平衡法具有更好的灵敏度和精密度。使用0.1 mL血浆时，非平衡法在低至0.2 ng/mL的可定量浓度下具有敏感性，并且也具有可重复性，相对标准偏差值通常小于±10%。在肌肉注射或静脉注射40 U/kg（8 μg/kg）依降钙素后，通过这种竞争性酶免疫分析法测定大鼠和犬的血浆水平。
