Shibata K, Takeda M, Ito A, Takeda M, Sagai H
Laboratory for Pharmacology, Institute for Life Science Research, Asahi Chemical Industry Co., Ltd., Ohito, Shizuoka, Japan.
Pharmacol Biochem Behav. 1998 Jun;60(2):371-6. doi: 10.1016/s0091-3057(98)00015-x.
Using ovariectomized (OVX) rats in the tail-withdrawal nociceptive test, we examined OVX-induced hyperalgesia and the antinociceptive effect of subcutaneously administered elcatonin, a synthetic derivative of eel calcitonin ([Asu1.7] eel calcitonin). Because tail-withdrawal latency was significantly and continuously reduced and bone mineral density decreased in OVX rats compared with those of sham-operated rats, it was demonstrated that ovariectomy induced prolonged hyperalgesia and osteoporosis. After repeated administrations for 3 or 4 weeks, subcutaneously injected elcatonin increased the latency of the OVX rats in a dose-dependent manner, compared to the vehicle-treated OVX rats. At a dose of 20 U/kg/day, there were significant differences (p < 0.01) in the latency between the elcatonin- and vehicle-treated OVX rats. This effect of elcatonin was completely inhibited by p-chlorophenylalanine treatment, suggesting that the central serotonergic system may be involved in the elcatonin antinociception of OVX-induced hyperalgesia.
在去卵巢（OVX）大鼠的甩尾伤害感受性试验中，我们检测了去卵巢诱导的痛觉过敏以及皮下注射鳗鱼降钙素的合成衍生物依降钙素（[Asu1.7]鳗鱼降钙素）的镇痛效果。与假手术大鼠相比，OVX大鼠的甩尾潜伏期显著且持续缩短，骨矿物质密度降低，这表明去卵巢诱导了长期的痛觉过敏和骨质疏松。在连续3或4周重复给药后，与给予赋形剂的OVX大鼠相比，皮下注射依降钙素使OVX大鼠的潜伏期呈剂量依赖性增加。在剂量为20 U/kg/天时，依降钙素处理组和赋形剂处理组的OVX大鼠在潜伏期上存在显著差异（p < 0.01）。对氯苯丙氨酸处理完全抑制了依降钙素的这种作用，这表明中枢5-羟色胺能系统可能参与了依降钙素对去卵巢诱导的痛觉过敏的镇痛作用。
