Baranowski Marcin, Górski Jan, Klapcinska Barbara, Waskiewicz Zbigniew, Sadowska-Krepa Ewa
Dept. of Physiology, Medical University of Bialystok, Bialystok, Poland.
Int J Sport Nutr Exerc Metab. 2014 Apr;24(2):148-56. doi: 10.1123/ijsnem.2013-0093. Epub 2013 Oct 2.
We have previously shown that acute exercise increases the level of sphingosine-1-phosphate (S1P) in plasma and ceramide in erythrocytes of untrained subjects. The aim of the current study was to examine the effect of ultramarathon run on the plasma and erythrocyte levels of the following bioactive sphingolipids: S1P, sphinganine-1-phosphate (SA1P), sphingosine, sphinganine, and ceramide. Blood samples were collected from seven male amateur runners participating in a 48-hr ultramarathon race before the run, after 24 and 48 hr of running, and following 24 and 48 hr of recovery. The sphingolipids were quantified by means of HPLC. Sustained running for 48 hr resulted in a progressive decline in plasma S1P to a level significantly lower than at prerace, and then remained stable over the next 48 hr of recovery. In erythrocytes, S1P content was stable until 24 hr of recovery, then rose abruptly to reach peak values after 48 hr of recovery. The plasma level of SA1P decreased progressively during the competition and remained unchanged over the recovery. In erythrocytes, the level of SA1P increased after 24 hr running and normalized thereafter. The level of ceramide, both in plasma and erythrocytes, was not significantly affected by the ultraendurance run. We speculate that reduction in plasma level of S1P during and after the run reduces its biological actions and might be responsible for some negative side-effects of the ultraendurance effort.
我们之前已经表明，急性运动可提高未经训练受试者血浆中鞘氨醇-1-磷酸（S1P）的水平以及红细胞中神经酰胺的水平。本研究的目的是检测超级马拉松跑对以下生物活性鞘脂的血浆和红细胞水平的影响：S1P、鞘氨醇-1-磷酸（SA1P）、鞘氨醇、二氢鞘氨醇和神经酰胺。从7名参加48小时超级马拉松比赛的男性业余跑步者身上采集血样，分别在跑步前、跑步24小时和48小时后以及恢复24小时和48小时后采集。通过高效液相色谱法对鞘脂进行定量分析。持续跑步48小时导致血浆S1P逐渐下降至显著低于赛前水平，然后在接下来的48小时恢复过程中保持稳定。在红细胞中，S1P含量在恢复24小时前保持稳定，然后在恢复48小时后突然上升至峰值。比赛期间血浆SA1P水平逐渐下降，恢复过程中保持不变。在红细胞中，跑步24小时后SA1P水平升高，此后恢复正常。超级耐力跑对血浆和红细胞中的神经酰胺水平均无显著影响。我们推测，跑步期间及之后血浆S1P水平的降低会减少其生物学作用，这可能是超级耐力运动产生一些负面副作用的原因。
